NMDA receptor N1 Antibody

Images

 
Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA ...read more
Flow Cytometry: NMDA receptor N1 Antibody [NB300-114] - CD4+ Lymphocytes expressing NMDAR1. Image provided by verified customer.

Product Details

Summary
Reactivity Mu, Rt, Hu, Ca, Ch, Pm, XpSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NMDA receptor N1 Antibody Summary

Immunogen
Peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor
Specificity
Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.
Predicted Species
Human (100%), Xenopus (100%), Primate (100%), Canine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:2000
Application Notes
NB 300-114 can be used in Western blot where a band is seen at ~ 100 kDa representing the NMDAR1 N1 splice variant.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB300-114 in the following applications:

Publications
Read Publication using NB300-114.

Reactivity Notes

It is anticipated that the antibody will also work with canine, chicken, human, non-human primate and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 50 ul PBS to desired concetration.

Background

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDA receptor N1 Antibody (NB300-114)(1)

Review for NMDA receptor N1 Antibody (NB300-114) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NB300-114:
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry NMDA receptor N1 NB300-114
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reviewed by:
Anonymous
Flow Mouse 01/31/2014
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Summary

ApplicationFlow Cytometry
Sample TestedBalb/c Mouse Lymphocytes
SpeciesMouse
LotCVP905YP

Blocking

Blocking DetailsN/A

Primary Anitbody

Dilution Ratio1:100 Dilution in FACS Buffer (PBS + 1% Fetal Bovine Serum)

Details

Detection NotesAcquired on FACSCaliber Flow Cytometer. Used Rabbit IgG Isotype Control as Negative Control
Fixation DetailsIncubated cells with Primary Antibody for 1 hour, washed with PBS 2x. Repeated with secondary antibody. Fixed with 4% PFA
Permeabilization DetailsN/A

Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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NMDA receptor N1 NB300-114

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Anonymous
01/31/2014
Application: Flow
Species: Mouse