NKX3.1 Antibody

Images

 
Western Blot: NKX3.1 Antibody [NB100-492] - analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. Image from verified customer review.
Western Blot: Nkx3.1 Antibody [NB100-492] - Analysis of Nkx3.1 using NB100-492 at 1:5000 on recombinant Nkx3.1 protein.
Western Blot: Nkx3.1 Antibody [NB100-492] - Detection of recombinant NKX3.1 protein (36 kDa) in WB assay using NB100-492. 250ng of purified recombinant HIS-tagged NKX3.1 full length protein was loaded onto a SDS-PAGE ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NKX3.1 Antibody Summary

Immunogen
A synthetic peptide made to residues 21-37 (TPSKPLTSFLIQDILRD) of human NKX3.1. This sequence is highly conserved in rat and mouse.
Specificity
This is specific for human NKX3.1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NKX3-1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody has been tested in Western blot. No other applications have been tested. Recommended dilution below. Western Blot - 1:1,000* However, the investigator should determine the optimal dilution. * This antibody has only been tested on recombinant protein.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NKX3.1 Lysate (NBL1-13662)
Reviewed Applications
Read 1 Review rated 5
using
NB100-492 in the following application:

Reactivity Notes

Human. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NKX3.1 Antibody

  • BAPX2
  • BAPX2homeobox protein Nkx-3.1
  • Homeobox protein NK-3 homolog A
  • NK homeobox (Drosophila), family 3, A
  • NK3 homeobox 1
  • NK3 transcription factor homolog A
  • NK3 transcription factor related, locus 1 (Drosophila)
  • NK3 transcription factor related, locus 1
  • NKX3.1
  • NKX3.1NKX3
  • NKX3-1
  • NKX3A
  • NKX3ANK homeobox, family 3, A

Background

NKX3.1 is a homeobox gene which exhibits prostate and testis specific expression. Loss of NKX3.1 expression has been implicated in prostate development and tumorigenesis. NKX3.1 expression is dramatically down-regulated in testicular cancer of germ cell origin. Results suggest that NKX3.1 is essential for normal testis function and that its loss of expression is highly associated with the invasive phenotype of testicular germ cell tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX3.1 Antibody (NB100-492) (0)

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Review for NKX3.1 Antibody (NB100-492) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-492:
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Images Ratings Applications Species Date Details
Western Blot NKX3.1 NB100-492
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reviewed by:
Ronald Miller
WB Human 11/20/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG - HRP (Life Tech)
Secondary Manufacturer Cat#65-6120
Secondary Concentration1:20K

Details

Detection NotesECL, 30 seconds exposure with film

Comments

CommentsTye antibody always give a weak band at higher molecular weight. Probably some cross reacting protein

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NKX3.1 Antibody (NB100-492) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Ronald Miller
11/20/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NKX3-1
Entrez