NKG2D/CD314 Antibody (CX5)

Images

 
Flow Cytometry: NKG2D/CD314 Antibody (CX5) [NBP1-43286] - Analysis using the Allophycocyanin conjugate of NBP1-43286. Staining of mouse splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE and 1.0 ug of Rat IgG1 ...read more
Flow Cytometry: NKG2D/CD314 Antibody (CX5) [NBP1-43286] - Two-color surface staining of mouse splenocytes with Anti-Mouse CD314 (NKG2D) PE and Anti-Mouse NK1.1 APC. Total viable cells were used for analysis.
Flow Cytometry: NKG2D/CD314 Antibody (CX5) [NBP1-43286] - Analysis using the PE conjugate of NBP1-43286. Analysis of mouse splenocyte suspensions using NKG2D PE. This can be used at less than or equal to 0.25 ug per ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43286
    • Availability
      Product Discontinued

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NKG2D/CD314 Antibody (CX5) Summary

Immunogen
The immunogen for this antibody was NKG2D CD314.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
KLRK1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
Application Notes
The CX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat. Other secondaries may give suboptimal staining.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for NKG2D/CD314 Antibody (CX5)

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1alpha, -beta, -gamma, -delta, -epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for NKG2D/CD314 Antibody (NBP1-43286)

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Bioinformatics

Gene Symbol KLRK1
Entrez