Nitrilase 1 Antibody (3A11)

Images

 
Western Blot: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nitrilase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Nitrilase 1 Antibody (3A11) [NBP2-02462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nitrilase 1.
Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Staining of paraffin-embedded pancreas tissue using anti-Nitrilase 1 mouse monoclonal antibody.
Flow Cytometry: Nitrilase 1 Antibody (3A11) [NBP2-02462] - HEK293T cells transfected with either pCMV6-ENTRY Nitrilase 1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Nitrilase 1 mouse ...read more
Immunocytochemistry/ Immunofluorescence: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Immunofluorescent staining of HT29 cells using anti-Nitrilase 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nitrilase 1 Antibody (3A11) [NBP2-02462] - Staining of paraffin-embedded Kidney tissue using anti-Nitrilase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3A11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Nitrilase 1 Antibody (3A11) Summary

Immunogen
Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NIT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nitrilase 1 Antibody (3A11)

  • EC 3.5
  • MGC57670
  • nitrilase 1
  • nitrilase homolog 1

Background

Play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nitrilase 1 Antibody (NBP2-02462) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NIT1
Entrez