NIT2 Antibody (2B9)

Images

 
Western Blot: NIT2 Antibody (2B9) [NBP2-02486] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: NIT2 Antibody (2B9) [NBP2-02486] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2.
Immunohistochemistry-Paraffin: NIT2 Antibody (2B9) [NBP2-02486] - Staining of paraffin-embedded pancreas tissue using anti-NIT2 mouse monoclonal antibody.
Flow Cytometry: NIT2 Antibody (2B9) [NBP2-02486] - HEK293T cells transfected with either pCMV6-ENTRY NIT2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT2 mouse monoclonal, and then ...read more
Immunohistochemistry-Paraffin: NIT2 Antibody (2B9) [NBP2-02486] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NIT2 Antibody (2B9) [NBP2-02486] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-NIT2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NIT2 Antibody (2B9) [NBP2-02486] - Staining of paraffin-embedded liver tissue using anti-NIT2 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NIT2 Antibody (2B9) Summary

Immunogen
Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NIT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NIT2 Antibody (2B9)

  • EC 3.5.1.3
  • MGC111199
  • Nit protein 2
  • nitrilase family, member 2
  • Nitrilase homolog 2
  • omega-amidase NIT2

Background

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NIT2
Entrez