NHERF-2 Antibody - BSA Free

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining in human spleen and tonsil tissues using anti-SLC9A3R2 antibody. Corresponding SLC9A3R2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining of human spleen shows high expression.

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NBP1-84944] - Staining of human tonsil shows low expression as expected.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NHERF-2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: RLVEVNGVNVEGETHHQVVQRIKAVEGQTRLLVVDQETDEELRRRQLTCTEEMAQRGLPPAHDPWEPKPDWAHTGSHSSEAGKKDVSGPLRELRPRLCHLRKGPQGYGFNLH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC9A3R2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  NHERF-2 Protein (NBP1-84944PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NHERF-2 Antibody - BSA Free

• E3KARP
• Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• NHE3RF2
• NHERF2MGC104639
• NHERF-2NHE3 kinase A regulatory protein E3KARP
• OCTS2
• SIP-1SRY-interacting protein 1
• sodium/hydrogen exchanger
• Sodium-hydrogen exchanger regulatory factor 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
• Solute carrier family 9 isoform A3 regulatory factor 2
• TKA-1SIP1
• Tyrosine kinase activator protein 1

Background

NHERF-2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A3R2
• Entrez:
  • 9351(Human)
• Uniprot:
  • Q15599(Human)