NHE3/SLC9A3 Antibody


Western Blot: NHE3 Antibody [NBP2-21844] - Antibody dilution 1:500 in antibody dilution buffer. Slc9A3 has an approximate MW of 44kDa

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NHE3/SLC9A3 Antibody Summary

Synthetic peptides (crlmpfrlssksvdsflqadgpeerppa) taken from Slc9A3 protein corresponding to 799-826 amino acid in located in the extended C-terminal domain. The peptide was covalently modified and was covalently coupled to a carrier protein by heterobifunctional cross linker for immunogen preparation
The antibody labels the Slc9A3 band of 44kda Slc9A3 protein in Western Blot positive control samples
Immunogen affinity purified
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  • Western Blot 1:500
  • Dot Blot 1:10000
  • ELISA 1:10000

Reactivity Notes

Rat, Mouse, Human and Monkey

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for NHE3/SLC9A3 Antibody

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3


The Solute Carrier proteins (SLC's) are membrane transport proteins that facilitate the transport of solute across plasma membrane (1). The SLC Family 9 proteins (SLC9A) are mainly responsible for sodium ion absorption by intestinal and renal epithelial tissue (2). The expression of Slc9A carrier proteins is sensitive to low levels of Na in the bloodstream. The SLC Family 9 proteins contain several isoforms that correspond to different loci on the SLC9 gene. There are eight distinct forms of the Solute Carrier protein, SLC9A1-A8 (5). These exchange proteins are localized to various regions of the small and large intestine, their expression varies throughout the gastrointestinal tract. The different isoforms of Slc9A genes also function distinctly from one another, the functional activities are sensitive to varying concentration of pharmacological antagonists. SLC9A3 plays a major role in exchange of Na and hydrogen ions and mediate intracellular signal transduction. Low levels of SLC9A proteins correspond to a reduced ability to absorb salt and water from digested chyme, often resulting in chronic diarrhea and bulky stools.
The Slc9A family members are Sodium-Hydrogen exchange proteins (Slc9As) and are located within the kidneys, stomach, and intestines, but the highest concentration of NHE exists within the large intestine. The levels of NHE vary drastically across the regions of the large intestines, with higher amounts of Slc9A1and Slc9A2 in the jejunum. Slc9A3 proteins are concentrated in the proximal colon, where they absorb Na/salt from the digested material prior to excretion (3). Intracellularly, Slc9A3 is located across the cell membrane of the colon epithelial cells, where Na+ ions pass through the channel protein for H+, which is later reabsorbed as water within the distal colon.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol SLC9A3