NGFI-B alpha/Nur77/NR4A1 Antibody (12.14)

Images

 
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (12.14) [NBP1-43269] - Immunoblotting of PMA and ionomycin-stimulated (lane 1) and unstimulated mouse thymocytes (lane 2) with 5 ug/mL of Anti-Mouse Nur77 Purified. The ...read more
Flow Cytometry: NGFI-B alpha/Nur77/NR4A1 Antibody (12.14) [NBP1-43269] - Analysis using the PE conjugate of NBP1-43269.
Flow Cytometry: NGFI-B alpha/Nur77/NR4A1 Antibody (12.14) [NBP1-43269] - Analysis using the PE conjugate of NBP1-43269. Staining of Nur77 in signaled thymocytes from B6 mouse. Dotted line is isotype control. Image from ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow
Clone
12.14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NGFI-B alpha/Nur77/NR4A1 Antibody (12.14) Summary

Immunogen
The immunogen for this antibody was Nur77.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NR4A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry
Application Notes
This 12.14 antibody has been tested by western blot on lysates prepared from mouse thymocytes stimulated with PMA and ionomycin for two hours. This antibody can be used at 5 ug/mL. It is recommended that this antibody by titrated for optimal performance in the assay of interest.

Reactivity Notes

This antibody is reactive to Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody (12.14)

  • Early response protein NAK1
  • GFRP1ST-59
  • growth factor-inducible nuclear protein N10
  • HMR
  • HMRNP10
  • hormone receptor
  • MGC9485
  • N10
  • NAK1
  • NAK-1
  • NGFIB alpha
  • NGFI-B alpha
  • NGFIB
  • NR4A1
  • Nuclear hormone receptor NUR/77
  • nuclear receptor subfamily 4 group A member 1
  • nuclear receptor subfamily 4, group A, member 1
  • Nur77
  • Orphan nuclear receptor HMR
  • Orphan nuclear receptor TR3
  • steroid receptor TR3
  • Testicular receptor 3
  • TR3 orphan receptor
  • TR3

Background

This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NR4A1
Entrez