NGF R/TNFRSF16/p75NTR Antibody

Images

 
Western Blot: NGF R/TNFRSF16/p75NTR Antibody [NB100-1539] - (0.3ug/ml) Staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NGF R/TNFRSF16/p75NTR Antibody Summary

Immunogen
Peptide with sequence C-AKREEVEKLLNG corresponding to internal region according to NP_002498.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NGFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 75 kDa band observed in human duodenum and human heart lysates (calculated MW of 45.2 kDa band according to NP_4804). The observed molecular weights correspond to earlier findings is reported in scientific literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1): 49-57).
Positive Control
Heart Lysate (NB820-59217)
p75NTR/NGF Receptor Lysate (NBL1-13635)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NGF R/TNFRSF16/p75NTR Antibody

  • CD271 antigen
  • CD271
  • Gp80-LNGFR
  • member 16
  • nerve growth factor receptor
  • NGF R
  • NGF receptor
  • NGFR
  • p75 ICD
  • p75 NTR
  • TNFRSF16
  • TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16)
  • tumor necrosis factor receptor superfamily member 16

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NGFR
Entrez
Uniprot