NFYA Antibody

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Western Blot: NFYA Antibody [NBP1-77819] - CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-77819
    • Availability
      Product Discontinued

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NFYA Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation and ion exchange chromatography.
Immunogen
Anti-NFYA antibody was produced by repeated immunizations with a synthetic NFYA peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFYA
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:10000
Application Notes
This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kDa forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] No reaction was observed by immunoblot against the 25 kDa B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] and ABTS substrate. A dilution of 1:10,000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for NFYA Antibody

  • CAAT box DNA-binding protein subunit A
  • CBF-A
  • CBF-B
  • CCAAT-binding transcription factor subunit B
  • FLJ11236
  • HAP2 CCAAT-binding protein
  • HAP2
  • NF-YACAAT-box DNA binding protein subunit A
  • Nuclear transcription factor Y subunit A
  • nuclear transcription factor Y subunit alpha
  • nuclear transcription factor Y, alpha
  • Transcription factor NF-Y, A subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NFYA