NFkB p105/p50 Antibody (4A11)

Images

 
Western Blot: NFkB p105/p50 Antibody (4A11) [H00004790-M02] - Analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody (4A11) [H00004790-M02] - Analysis of monoclonal antibody to NFKB1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (4A11) [H00004790-M02] - Analysis of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml.
Proximity Ligation Assay: NFkB p105/p50 Antibody (4A11) [H00004790-M02] - Analysis of protein-protein interactions between HSP90AB1 and NFKB1. HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 ...read more
Sandwich ELISA: NFkB p105/p50 Antibody (4A11) [H00004790-M02] - Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00004790-M02
    • Availability
      Product Discontinued

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NFkB p105/p50 Antibody (4A11) Summary

Immunogen
NFKB1 (AAH51765 860 a.a. - 969 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
Specificity
NFKB1 - nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKB1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFkB p105/p50 Antibody (4A11)

  • DKFZp686C01211
  • DNA binding factor KBF1
  • DNA-binding factor KBF1
  • EBP-1
  • KBF1
  • NF-kappaB
  • NF-kappa-B
  • NF-kappabeta
  • NFKB-p105
  • NFKB-p50
  • nuclear factor kappa-B DNA binding subunit
  • nuclear factor NF-kappa-B p105 subunit
  • nuclear factor NF-kappa-B p50 subunit
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
  • p105
  • p50

Background

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for NFkB p105/p50 Antibody (H00004790-M02)

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Research Areas for NFkB p105/p50 Antibody (H00004790-M02)

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Blogs on NFkB p105/p50.

Nuclear Factor kappa B Signaling in the Immune System
Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed...  Read full blog post.

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Bioinformatics

Gene Symbol NFKB1
Entrez
OMIM
Uniprot