NF-M Antibody

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Western Blot: NF-M Antibody [NBP1-45213] - (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-45213
    • Availability
      Product Discontinued

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NF-M Antibody Summary

Immunogen
Peptide with sequence C-QASHITVERKDYLK corresponding to internal region according to NP_001099011.1.
Epitope
internal region
Marker
Neuronal Marker
Specificity
This antibody is expected to recognize both reported isoforms (NP_005373.2; NP_001099011.1).
Clonality
Polyclonal
Host
Goat
Gene
NEFM
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
Publications
Read Publication using NBP1-45213.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse,Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NF-M Antibody

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NF-M Antibody (NBP1-45213)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEFM
Entrez
Uniprot