NF-L Antibody

Images

 
Western Blot: 68kDa Neurofilament Light Antibody [NBP2-14866] - Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 7.5% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: 68kDa Neurofilament Light Antibody [NBP2-14866] - Paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-14866
    • Availability
      Product Discontinued

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NF-L Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human 68kDa Neurofilament Light. The exact sequence is proprietary.
Marker
Neuronal Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEFL
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional NF-L Products

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Bioinformatics

Gene Symbol NEFL
Entrez