NF-L Antibody

Images

 
Western Blot: NF-L Antibody [H00004747-B01P] - Analysis of NEFL expression in rat brain.
Western Blot: NF-L Antibody [H00004747-B01P] - Analysis of NEFL expression in transfected 293T cell line by NEFL polyclonal antibody. Lane 1: NEFL transfected lysate(59.73 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00004747-B01P
    • Availability
      Product Discontinued

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NF-L Antibody Summary

Immunogen
NEFL (NP_006149.2, 1 a.a. - 543 a.a.) full-length human protein. MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Marker
Neuronal Marker
Clonality
Polyclonal
Host
Mouse
Gene
NEFL
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
It has been used for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NF-L Antibody

  • 68 kDa neurofilament protein
  • CMT2E
  • NEFL
  • neurofilament protein, light chain
  • neurofilament subunit NF-L
  • Neurofilament triplet L protein
  • neurofilament, light polypeptide
  • neurofilament-light
  • NF68
  • NF68FLJ53642
  • NFL
  • NF-L
  • NFLlight polypeptide 68kDa

Background

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tail piece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. This antibody provides an early marker of neuronal migration and differentiation in human fetal development yielding Golgi like images without the disadvantages of lack of selectivity and poor specificity of the Golgi technique. In specifically delineating cell bodies and dendrites, this antibody can be used to trace the inside out gradient of neuron production and differentiation. Pathologic conditions, such as under nutrition affect this antibody visualized soma size and dendritic arborization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEFL
Entrez
Uniprot