Neurotensin Receptor 2 Antibody

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Western Blot: Neurotensin Receptor 2 Antibody [NBP1-00959] - Western blot (WB) analysis of NTR2 (D184) antibody in extracts from Jurkat cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00959
    • Availability
      Product Discontinued

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Neurotensin Receptor 2 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 151-200 of Human NTR2.
Clonality
Polyclonal
Host
Rabbit
Gene
NTSR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of NTR2 (D184) pAb in extracts from Jurkat cells.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurotensin Receptor 2 Antibody

  • neurotensin receptor 2
  • neurotensin receptor type 2
  • neurotensin receptor, type 2
  • NT-R-2
  • NTR2Levocabastine-sensitive neurotensin receptor

Background

Neurotensin (NT) initiates an intracellular response by interacting with the G protein-coupled receptors NTR1 (NTS1 receptor, high affinity NTR) and NTR2 (NTS2 receptor, levocabastine-sensitive neurotensin receptor), and the type I receptor NTR3 (NTS3 receptor, sortilin-1, Gp95). NT has a wide distribution in regions of the brain and in peripheral tissues where NT receptors can contribute to hypotension, hyperglycemia, hypothermia, antinociception and regulation of intestinal motility and secretion. HL-60 cells express NTR1, which can couple to Gq, Gi/o or Gs. Alternative splicing of rat NTR2 can generate a 5-transmembrane domain variant isoform that is coexpressed with the full length NTR2 throughout the brain and spinal cord. NTR3 activation in the murine microglial cell line N11 induces MIP-2, MCP-1, IL-1beta and TNFalpha in an ERK1/2 and Akt kinase-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NTSR2
Entrez