Neuropilin-1 Antibody

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Western Blot: Neuropilin-1 Antibody [NBP1-45984] - Western blot analysis of Neuropilin-1 in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-45984
    • Availability
      Product Discontinued

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Neuropilin-1 Antibody Summary

Immunogen
Synthetic peptide surrounding amino acid 816 of mouse Neuropilin 1.
Clonality
Polyclonal
Host
Rabbit
Gene
NRP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~100-130 kDa band of Neuropilin 1 in samples from human, mouse and rat, origins. A lower band ~50-70 kDa can also be detected, presumably to be the cleavage fragment of Neuropilin-1. Reactivity to other species has not been tested.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Neuropilin-1 Antibody

  • BDCA4
  • BDCA-4
  • CD304 antigen
  • CD304
  • DKFZp686A03134
  • DKFZp781F1414
  • neuropilin 1
  • Neuropilin1
  • Neuropilin-1
  • NRP1
  • NRPNP1
  • transmembrane receptor
  • Vascular endothelial cell growth factor 165 receptor
  • VEGF165RCD304

Background

The Neuropilin-1 antibody reacts with mouse Neuropilin-1, a cell surface glycoprotein originally identified in Xenopus tadpole nervous tissues. Immunohistochemistry and in situ hybridization analyses in mice indicated that the expression of neuropilin was restricted to axons of particular neuron classes, and at stages when axon growth is active. Neuropilin protein was localized on axons but not on the somata of neurons and can mediate cell adhesion by heterophilic molecular interaction. The gain and loss of function of neuropilin in developing mouse embryos causes defasciculation and incorrect sprouting of nerve fibers. It is postulated that neuropilin serves in a variety of neuronal cell interactions by binding to a variety of molecules, and that it plays essential roles in nerve fiber fasciculation and targeting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Neuropilin-1 Antibody (NBP1-45984)

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Diseases for Neuropilin-1 Antibody (NBP1-45984)

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PTMs for Neuropilin-1 Antibody (NBP1-45984)

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Bioinformatics

Gene Symbol NRP1
Entrez
Uniprot