Neuronostatin Antibody (2956E) [Unconjugated]

Neuronostatin was detected in fixed TT human medullary thyroid cancer cell line (Positive) and absent in THP‑1 human acute monocytic leukemia cell line (Negative) using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Neuronostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western shows lysates of Neuronostatin Peptide SML0354, loaded at 200 ng/ml. Specific bands were detected for Neuronostatin at approximately 2-4 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human Neuronostatin Monoclonal Antibody (Catalog # MAB11554). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

• Reactivity: Hu
• Applications: Simple Western, IHC, ICC/IF
• Clone: 2956E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Neuronostatin Antibody (2956E) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human Neuronostatin containing synthetic peptide
• Specificity: Detects a peptide specific for Neuronostatin in a region around amino acid 95 in Direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-10 ug/mL
  • Immunohistochemistry 1-10 ug/mL
  • Simple Western 20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuronostatin Antibody (2956E) [Unconjugated]

• Neuronostatin
• Neuronostatin-13
• NST
• preprosomatostatin
• prepro-somatostatin

Background

Neuronostatin is a 13-amino acid bioactive peptide hormone encoded by the somatostatin gene.  Neuronostatin has a widespread effect in the CNS and peripheral tissues.  It reduces food and water intake, increases mean arterial pressure, delays gastrointestinal transit, and increases glucagon secretion from pancreatic islet alpha cells among other things.  Neuronostatin is produced in the endocrine pancreas, specifically pancreatic beta cells. The amounts of immunoreactive neuronostatin are highest in spleen, pancreas, cerebrum, and hypothalamus but is also found in diverse tissues including the heart.

1. Yang S, Zhao X, Du Y, Yu P. Emerging functions of neuronostatin in physiology, pathology, and potential therapeutics. Neuropeptides. 2022 Aug;94:102257. doi: 10.1016/j.npep.2022.102257. Epub 2022 May 20. PMID: 35660860.
2. Wei D, Sun Y. Krążek M, Ligęza A, Wojciechowicz T, Skrzypski M. Biologiczna rola neuronostatyny, hormonu kodowanego przez gen somatostatyny [The biological role of neuronostatin, a hormone encoded by the somatostatin gene]. Postepy Biochem. 2023 Jun 5;69(2):89-95. Polish. doi: 10.18388/pb.2021_476. PMID: 37493559. 
3. Salvatori AS, Elrick MM, Samson WK, Corbett JA, Yosten GL. Neuronostatin inhibits glucose-stimulated insulin secretion via direct action on the pancreatic alpha -cell. Am J Physiol Endocrinol Metab. 2014 Jun 1;306(11):E1257-63. doi: 10.1152/ajpendo.00599.2013. Epub 2014 Apr 15. PMID: 24735892; PMCID: PMC4042099.
4. Vainio L, Perjes A, Ryti N, Magga J, Alakoski T, Serpi R, Kaikkonen L, Piuhola J, Szokodi I, Ruskoaho H, Kerkelä R. Neuronostatin, a novel peptide encoded by somatostatin gene, regulates cardiac contractile function and cardiomyocyte survival. J Biol Chem. 2012 Feb 10;287(7):4572-80. doi: 10.1074/jbc.M111.289215. Epub 2011 Dec 14. PMID: 22170057; PMCID: PMC3281621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.