Neurogenin-3 Antibody (3B5)

Images

 
Western Blot: Neurogenin-3 Antibody (3B5) [NBP1-47878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: Neurogenin-3 Antibody (3B5) [NBP1-47878] - Neurogenin3 Immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.
Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (3B5) [NBP1-47878] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (3B5) [NBP1-47878] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Neurogenin-3 Antibody (3B5) Summary

Immunogen
Protein expressed in 293T cell transfected with human NEUROG3 expression vector
Specificity
This antibody is specific for Homo sapiens neurogenin 3 (NEUROG3).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NEUROG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50 - 1:150
  • Immunohistochemistry-Paraffin 1:50 - 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Neurogenin-3 Antibody (3B5)

  • Atoh5
  • BHLHA7
  • bHLHa7NGN-3
  • Class A basic helix-loop-helix protein 7
  • Math4B
  • NEUROG3
  • neurogenin 3
  • Neurogenin3
  • Neurogenin-3
  • NGN3
  • Protein atonal homolog 5
  • Relax

Background

Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NEUROG3
Entrez