NETO1 Antibody (532509) [Unconjugated]

• Reactivity: Hu
• Applications: IHC
• Clone: 532509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NETO1 Antibody (532509) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human NETO1 isoform 3
  Thr23-Val345
  Accession # Q8TDF5
• Specificity: Detects human NETO1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NETO1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NETO1 Antibody (532509) [Unconjugated]

• BCTL1
• Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 1
• BTCL1
• BTCL1BCTL1neuropilin and tolloid-like protein 1
• FLJ41325
• NETO1
• neuropilin (NRP) and tolloid (TLL)-like 1

Background

Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina. The 511 amino acid residue mature human NETO1 contains an extracellular domain with two CUB domains (aa 41‑155 and 172‑287), and one class A LDL-receptor segment (aa 292‑326). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation. Over aa 23‑345, human NETO1 shares 97% and 98% aa identity with mouse and canine NETO1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NETO1
• Uniprot:
  • Q8TDF5()