NEK9 Antibody

Product Discontinued

NEK9 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 170-220 of Human Nek9.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Nek9 (S206) pAb in extracts from A549 cells.
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEK9 Antibody

• DKFZp434D0935
• KIAA1995
• MGC138306
• MGC16714
• Nek8
• Nek8NERCC1
• NEK9
• NERCC
• Nercc1 kinase
• NERCCEC 2.7.11.1
• Never in mitosis A-related kinase 9
• NIMA (never in mitosis gene a)- related kinase 9
• NimA-related kinase 8
• NIMA-related kinase Nek8
• NimA-related protein kinase 9
• serine/threonine-protein kinase Nek9

Background

Nek8 (also designated never in mitosis A (NimA)-related kinase 8, Jck and NEK12A) is a mitotic regulator that plays a significant role in maintaining renal tubular integrity. Nek8 transcript is abundant in thyroid, adrenal gland and skin, with overexpression in breast tumors and infiltrating ductal carcinomas, and moderate levels in mucinous adenocarcinoma. Nek8 mutations correlate to juvenile autosomal recessive polycystic kidney disease in humans. Nek9 (never in mitosis A (NimA)-related kinase 9, Nercc1 kinase) regulates G1/S transition and S phase progression by influencing spindle dynamics and chromosome separation. Nek9 phosphorylates different histones (Histone H3 on serine and threonine), myelin basic protein, beta-casein (serine) and BICD2. Nek9 interacts with Ran GTPase, Nek6, Nek7, BICD2, SSRP1 and SUPT16H/ FACT complex. Nek9 transcript is abundant in heart, liver, kidney and testis. Nek family cell-cycle protein kinases in humans have at least 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus nidulans kinase that plays on several aspects of mitosis, with divergent carboxyl-terminal tails of varying length.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK9
• Entrez:
  • 91754(Human)
  • 217718(Mouse)
  • 299204(Rat)