NEK9 [p Thr210] Antibody

Product Discontinued

NEK9 [p Thr210] Antibody Summary

• Immunogen: Partial recombinant protein, responding to the middle region of NEK-9
• Modification: p Thr210
• Specificity: NEK9 [Thr210]
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NEK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Application Notes: IHC & IP reported in the literature. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NEK9 Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NEK9 [p Thr210] Antibody

• DKFZp434D0935
• KIAA1995
• MGC138306
• MGC16714
• Nek8
• Nek8NERCC1
• NEK9
• NERCC
• Nercc1 kinase
• NERCCEC 2.7.11.1
• Never in mitosis A-related kinase 9
• NIMA (never in mitosis gene a)- related kinase 9
• NimA-related kinase 8
• NIMA-related kinase Nek8
• NimA-related protein kinase 9
• serine/threonine-protein kinase Nek9

Background

NEK9 is a 107 kD member of the serine/threonine-protein kinase family, NIMA subfamily containing a coiled-coil domain, and RCC1 repeats. This cytoplasmic protein forms dimers and is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation, Nek9 is activated during mitosis by intramolecular autophosphorylation at Thr210. Nek9 has been shown to interact with Ran GTPase (greater affinity for Ran-GDP over Ran-GTP), NEK6, NEK7, BICD2, histone H3, myelin basic protein, and beta-casein. The Poly6241 antibody recognizes human NEK9 and has been shown to be useful for Western blotting, immunohistochemistry, and immunoprecipitation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEK9
• Entrez:
  • 91754(Human)
• Uniprot:
  • Q8TD19(Human)