NEK9 Antibody

Images

 
Western Blot: NEK9 Antibody [NB100-58818] - Detection of Human and Mouse NEK9 on HeLa whole cell lysate using NB100-58818. NEK9 was also immunoprecipitated by rabbit anti-NEK9 antibody NB100-58817.
Proximity Ligation Assay: NEK9 Antibody [NB100-58818] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human NEK9 (NB100-58818). Image merged from DAPI (2ms), ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-58818
    • Availability
      Product Discontinued

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NEK9 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 929 and 979 of human NIMA related kinase 9 using the numbering given in entry NP_149107.3 (GeneID 91754).
Clonality
Polyclonal
Host
Rabbit
Gene
NEK9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
Application Notes
Western Blot - 1:2,000 to 1:10,000. Immunoprecipitation - 2 to 5 mcg/mg lysate. Immunohisto-/cytochemistry - Not tested.
Positive Control
NEK9 Lysate (NBL1-13588)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NEK9 Antibody

  • DKFZp434D0935
  • KIAA1995
  • MGC138306
  • MGC16714
  • NEK8
  • Nek8NERCC1
  • Nercc1 kinase
  • NERCCEC 2.7.11.1
  • Never in mitosis A-related kinase 9
  • NIMA (never in mitosis gene a)- related kinase 9
  • NimA-related kinase 8
  • NIMA-related kinase Nek8
  • NimA-related protein kinase 9
  • serine/threonine-protein kinase Nek9

Background

NEK9 is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. NEK9 phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional NEK9 Products

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Bioinformatics

Gene Symbol NEK9
Entrez
Uniprot