NDUFS3 Antibody - BSA Free

Western Blot: NDUFS3 Antibody [NBP2-19505] - Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: NDUFS3 Antibody [NBP2-19505] - . Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.

Immunohistochemistry-Paraffin: NDUFS3 Antibody [NBP2-19505] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Western Blot: NDUFS3 Antibody [NBP2-19505] - Sample (30 ug of whole cell lysate) A: THP-1 B: HL-60 12% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, RM
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFS3 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit NDUFS3 Antibody - BSA Free (NBP2-19505) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane
• Predicted Species: Mouse (92%), Rat (91%), Porcine (93%), Bovine (94%), Rhesus Macaque (98%), Chicken (90%), Primate (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (96%), Zebrafish (81%), Xenopus laevis (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NDUFS3 Antibody - BSA Free

• CI-30
• CI-30kD
• complex I 30kDa subunit
• Complex I-30kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
• NADH dehydrogenase-ubiquinone 30 kDa subunit
• NADH-ubiquinone oxidoreductase 30 kDa subunit

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFS3
• Uniprot:
  • O75489(Human)