NDUFA5 Antibody (1E8)

Images

 
Western Blot: NDUFA5 Antibody (1E8) [NBP2-01454] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: NDUFA5 Antibody (1E8) [NBP2-01454] Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDUFA5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
1E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NDUFA5 Antibody (1E8) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 3-116 of human NDUFA5(NP_004991) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NDUFA5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NDUFA5 Antibody (1E8)

  • B13,5 (13kD, B13)
  • DKFZp781K1356
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
  • UQOR13FLJ12147

Background

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NDUFA5
Entrez