NCOA4 Antibody



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NCOA4 Antibody Summary

Synthetic peptide, corresponding to amino acids 348-398 of Human ARA70.
ARA70 (L379) pAb detects endogenous levels of ARA70 protein.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of ARA70 (L379) pAb in extracts from 293 cells.
Immunohistochemistry (IHC) analyzes of ARA70 (L379) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NCOA4 Lysate (NBP2-64955)

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for NCOA4 Antibody

  • Androgen receptor coactivator 70 kDa protein
  • Androgen receptor-associated protein of 70 kDa
  • ARA70RFGret fused
  • DKFZp762E1112
  • ELE1NCoA-4
  • nuclear receptor coactivator 4,70 kDa AR-activator
  • PTC3RET-activating gene ELE1
  • Ret-activating protein ELE1,70 kDa androgen receptor coactivator


Androgen receptor (AR) co-activator ARA70, also designated RFG and ELE1,is a putative co-activator that specifically enhances the activity of the ndrogen receptor. In human thyroid carcinomas, the Ret proto-oncogene fuses to ARA70 to form Ret/PTC3 by an intrachromosomal inversion of chromosome 10 in vivo. ARA70 is expressed as two isoforms, ARA70 alpha and ARA70 beta. The shorter variant, ARA70 beta, results from an internal 985-bp deletion. ARA70 alpha is widely expressed, and its expression is highest in testis and adipose tissues,whereas ARA70 beta is solely expressed in testis. ARA70 alpha can function as a ligand-enhanced co-activator of PPAR gamma in adipocytes. However, PPAR gamma -ARA70 transactivation can be squelched by AR, which suggests cross talk between PPAR gamma - and AR-mediated response. ARA70 alpha has no intrinsic transcription activation domain or histone acetyltransferase activity, but it interacts with histone acetyltransferase, p/CAF, CBP and p300/CBP-associated factors, and the basal transcription factor TFIIB. The interaction between ARA70 and AR occurs through the ligand-binding domain. The presence of ARA70 can enhance the androgenic activity of 17-beta Estradiol (E2) and antiandrogens toward AR. ARA70 may be involved in prostate carcinogenesis and ovarian cancer, and may serve as a key mediator of estrogen-androgen synergism.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol NCOA4