Nbs1 Antibody (Y112)

Product Discontinued

Nbs1 Antibody (Y112) Summary

• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p95 NBS1 aa 650-750 (C terminal).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NBN
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 1:60
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 95 kDa. Immunofluorescence (PMID: 21470188)
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Nbs1 Overexpression Lysate

Reactivity Notes

Cross reactivity determined by western blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS (pH 7.2), 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Nbs1 Antibody (Y112)

• ATV
• AT-V1
• AT-V2
• Cell cycle regulatory protein p95
• FLJ10155
• MGC87362
• NBN
• NBS
• Nbs1
• NBS1P95
• Nibrin
• Nijmegen breakage syndrome 1 (nibrin)
• Nijmegen breakage syndrome protein 1
• p95 protein of the MRE11/RAD50 complex
• p95

Background

NBS1 (Nijmegen breakage syndrome protein 1, Nibrin) is the eukaryotic component of MRN complex (Mre11-Rad50-Nbs1) involved in homologous recombination repair for DNA double-strand breaks (DSBs) and DNA damage-induced checkpoint activation. NBS1, a nuclear protein with a theoretical molecular weight of 65-85 kDa, is composed of 2 functional regions: the forkhead associated (FHA) N-terminal domain (24-83 amino acids) and the breast cancer C-terminus (BRCT) domain (105-181 amino acids). Mutations in the NBN gene are associated with Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and an increased susceptibility to prostate cancer, lung cancer, liver cancer and intrahepatic cholangiocarcinoma (IHC) (1).

In DNA double strand break repair, the FHA/BRCT domains bind DNA damage sensor proteins including gamma H2AX (phospho Ser139), phosphorylated MDC1 and CtIP (CtBP-interacting protein). NBS1 then recruits the other members of the MRN complex, Mre11 and Rad50, to the proximity of DNA DSBs. The MRN complex has also been shown to interact with ATM or ATR kinases in the presence of DSBs or replication fork stalling, respectively. Phosphorylation of NBS1 at Ser 278 and Ser343 in response to ionizing radiation (IR) is dependent on ATM and is important for activation of the S phase checkpoint. The activation of ATM in response to DNA damage is also facilitated by MRN and may lead to induction of apoptosis (2, 3).

References

1. Bian L, Meng Y, Zhang M, Li D. (2019) MRE11-RAD50-NBS1 complex alterations and DNA damage response: implications for cancer treatment. Mol Cancer. 26;18(1):169. PMID: 31767017

2. Zhang Y, Zhou J, Lim CU. (2006) The role of NBS1 in DNA double strand break repair, telomere stability, and cell cycle checkpoint control. Cell Res. 16(1):45-54. PMID: 16467875

3. Komatsu K. NBS1 and multiple regulations of DNA damage response. (2016) J Radiat Res. 57 Suppl 1:i11-i17. PMID: 27068998

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nbs1 Antibody (NB110-57272)(8)

Publications using NB110-57272

• Chen G, Zhang B, Xu H et al. Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis Oncogene 2017-09-04 [PMID: 28869605] (WB, Human)
• Hartlerode AJ, Morgan MJ, Wu Y et al. Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors. Nat. Struct. Mol. Biol. 2015-08-17 [PMID: 26280532] (WB, Mouse)
• Mason JM, Das I, Arlt M et al. The SNM1B/APOLLO DNA nuclease functions in resolution of replication stress and maintenance of common fragile site stability. Hum Mol Genet 2013-07-30 [PMID: 23863462] (ICC/IF, Human)
• Bensimon A, Schmidt A, Ziv Y et al. ATM-dependent and -independent dynamics of the nuclear phosphoproteome after DNA damage. Sci Signal 2010-12-01 [PMID: 21139141]
• Uhl M, Csernok A, Aydin S et al. Role of SIRT1 in homologous recombination. DNA Repair (Amst) 2010-04-01 [PMID: 20097625] (WB)
• Momcilovi? O, Choi S, Varum S et al. Ionizing radiation induces ataxia telangiectasia mutated-dependent checkpoint signaling and G(2) but not G(1) cell cycle arrest in pluripotent human embryonic stem cells. Stem Cells 2009-08-01 [PMID: 19544417]
• Smith CJ. Mechanisms in suppressing chromosomal translocations and maintaining genome stability. Clinical Colorectal Cancer. 2014-09-21 (WB, Mouse)
  
  Details:
  NBS1 antibody used for WB in experiments involving Artemis+/+ and ArtemisP70/P70 mouse embryonic fibroblasts
• Blickwedehl J, Agarwal M, Seong C et al. Role for proteasome activator PA200 postglutamyl proteasome activity in genomic stability. PNAS;105(42):16165-16170. 2008-01-01 [PMID: 18845680]

Review for Nbs1 Antibody (NB110-57272) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: HEK293 whole cell lysate
• Species: Human

FAQs for Nbs1 Antibody (NB110-57272). (Showing 1 - 6 of 6 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • Yes, if this product is used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product.
2. What research areas can this product be used in?
   • All Nbs1 products can be used in Breast Cancer, Cancer, Checkpoint signaling, Chromatin Research, DNA Double Strand Break Repair, DNA Repair, Homologous Recombination, Non-homologous end-joining, Tumor Suppressors, and Cell Cycle and Replication.
3. What is NBS1?
   • NBS1 is Nijmegen breakage syndrome protein 1, or Nibrin. Please refer to the background listed in the datasheet for a full summary of this common name.
4. What is the theoretical molecular weight of Nbs1 antibodies?
   • In general, there is only one isoform described for this target. The TMW of the human form of the target protein is 85kDa. The mouse form is 84kDa. However, the observed molecular weight may vary.
5. We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?
   • I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.
6. I was wondering what the antibody concentration would be for nbs1 (NB110-57272) lot 1033101c?
   • The concentration is measured for unpurified tissue culture supernatants, since our antibodies contain rabbit IgG. For lot# YI033101C, the IgG concentration is 0.503 mg/mL.

Bioinformatics

• Gene Symbol: NBN
• Entrez:
  • 4683(Human)
  • 27354(Mouse)
  • 85482(Rat)
• Uniprot:
  • O60934()