Nanog Antibody

Images

 
Western Blot: Nanog Antibody [NBP1-50402] - Analysis of anti-NANOG antibody with HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: Nanog Antibody [NBP1-50402] - Analysis of anti-NANOG antibody with human testis at concentration 2 ug/ml.

Product Details

Summary
Product Discontinued
View other related Nanog Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50402
    • Availability
      Product Discontinued

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Nanog Antibody Summary

Immunogen
Synthetic peptide C-DLFLNYSMNMQPED from the C-terminus of human NANOG (NP_079141.2).
Marker
Embryonic Stem Cell Marker
Specificity
Human NANOG.
Clonality
Polyclonal
Host
Goat
Gene
NANOG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.1 ug/ml
  • ELISA 1:128000
  • Immunohistochemistry-Paraffin 2 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (93%), Gorilla (93%), Monkey (86%), Marmoset (86%), Tamarin (86%), Porcine (86%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nanog Antibody

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nanog Antibody (NBP1-50402) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Nanog Antibody (NBP1-50402) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Nanog Products

Bioinformatics Tool for Nanog Antibody (NBP1-50402)

Discover related pathways, diseases and genes to Nanog Antibody (NBP1-50402). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nanog Antibody (NBP1-50402)

Discover more about diseases related to Nanog Antibody (NBP1-50402).
 

Pathways for Nanog Antibody (NBP1-50402)

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PTMs for Nanog Antibody (NBP1-50402)

Learn more about PTMs related to Nanog Antibody (NBP1-50402).

Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot