Nanog Antibody (hNanog.2)

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Western Blot: Nanog Antibody (hNanog.2) [NBP1-43304] - NTERA cell line lysate was loaded at 1x10^5 cells/lane, probed with 1 ug/mL of Anti-Human Nanog Purified and revealed with Anti-Mouse IgG HRP.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43304
    • Availability
      Product Discontinued

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Nanog Antibody (hNanog.2) Summary

Immunogen
Human Nanog
Marker
Embryonic Stem Cell Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NANOG
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This Nanog antibody has been tested by immunoblotting of NTERA cell lysates.

Reactivity Notes

This antibody is reactive to Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Nanog Antibody (hNanog.2)

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

The hNanog.2 antibody was generated against and reacts with human Nanog, a homeobox transcription factor. In addition to the known Oct4 and Stat3 transcriptional operating systems, Nanog plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. Nanog was identified as a gene specifically expressed in mouse ES cells and preimplantation embryos and is capable of maintaining ES cell self-renewal independently of LIF/Stat3. Potential cross-reactivity of this antibody to other proteins has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Nanog Products

Bioinformatics Tool for Nanog Antibody (NBP1-43304)

Discover related pathways, diseases and genes to Nanog Antibody (NBP1-43304). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nanog Antibody (NBP1-43304)

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Pathways for Nanog Antibody (NBP1-43304)

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PTMs for Nanog Antibody (NBP1-43304)

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Research Areas for Nanog Antibody (NBP1-43304)

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol NANOG
Entrez