n-Myc Antibody

ELISA: n-Myc Antibody [23960002]

Product Discontinued

n-Myc Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: Human MYCN.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYCN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoassay
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Use in immunoassay reported in scientific literature (PMID 26686971). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30349086).

Reactivity Notes

Human. Use in Mouse reported in scientific literature (PMID:30182136).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for n-Myc Antibody

• BHLHE37
• bHLHe37N-myc proto-oncogene protein
• Class E basic helix-loop-helix protein 37
• MODED
• neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
• N-myc
• NMYCneuroblastoma MYC oncogene
• ODED
• oncogene NMYC
• pp65/67
• v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
• v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for n-Myc Antibody (23960002)(7)

Publications using 23960002

• Ognibene M, Podesta M, Garaventa A, Pezzolo A Role of GOLPH3 and TPX2 in Neuroblastoma DNA Damage Response and Cell Resistance to Chemotherapy Int J Mol Sci 2019-09-25 [PMID: 31557970] (WB, Human)
• Brabetz S, Leary SES, Grobner SN et al. A biobank of patient-derived pediatric brain tumor models. Nat. Med. 2018-10-22 [PMID: 30349086] (IHC-P, Human)
• Brandl L, Hartmann D, Kirchner T, Menssen A. Expression of n-MYC, NAMPT and SIRT1 in Basal Cell Carcinomas and their Cells of Origin. Acta Derm. Venereol. 2018-09-05 [PMID: 30182136] (IF/IHC, Human, Mouse)
• Ognibene M, Cangelosi D, Morini M et al. Immunohistochemical analysis of PDK1, PHD3 and HIF-1 alpha expression defines the hypoxic status of neuroblastoma tumors PLoS One. 2017-11-07 [PMID: 29117193] (WB, IF/IHC, Human)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Smith JR, Moreno L, Heaton SP et al. Novel pharmacodynamic biomarkers for MYCN protein and PI3K/AKT/mTOR pathway signaling in children with neuroblastoma. Molecular Oncology. 2015-11-19 [PMID: 26686971] (IA, Human)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

FAQs for n-Myc Antibody (23960002). (Showing 1 - 1 of 1 FAQs).

1. Our customer needs to have the protocol for the IHC paraffin application of this antibody. Is a demasking protocol required? If yes, which one: enzimatic or microwave pretreatment ?
   • We recommend citrate buffer at a pH of 6.0 for antigen retrieval for all of our antibodies.

Bioinformatics

• Gene Symbol: MYCN
• Entrez:
  • 4613(Human)
• Uniprot:
  • P04198(Human)