Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody [NB200-592] - A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), ...read more
Immunohistochemistry: N-Cadherin Antibody [NB200-592] - Section of human heart.
Synthetic peptide: DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH by a Glutaraldehyde linker. Sequence corrresponds to the C-terminal amino acids of N-Cadherin (Chicken), with an extra N-terminal lysine residue (24 amino acids).
Mesenchymal Cells Marker
Recognizes all cadherin members. Rabbit Anti-Pan Cadherin shows specific reactivity with a distinct 135 kDa band on chicken or rabbit heart extract blots using indirect immunoblotting.
Test in a species/application not listed above to receive a full credit towards a future purchase.
This product is unpurified. The exact concentration of antibody is not quantifiable.
This antibody is useful for: Western Blot. IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: On chicken and rabbit heart extract this antibody detects a single band at 135 kD.
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca++-dependent cell-cell adhesion.1,2,3 The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually, a cytoplasmic C-terminal tail4 which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and a-actinin. Cadherins which are primarily located in areas of cell-cell contacts, are involved in selective cell sorting and in the mechanical cytoplasmic response. They are implicated in morphogenetic processes, intercellular signalling and tumor invasiveness and metastasis. Multiple cadherins were characterized from diverse species and tissues including E-Cadherin, N-Cadherin (A-CAM), P-Cadherin, V-Cadherin, R-Cadherin and T-Cadherin. Specific antibodies against a highly conserved sequence from the cytoplasmic C-terminal of N-Cadherin have been prepared.5,6
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.