Myosin Skeletal Antibody (NOQ7.5.4D)

Images

 
Western Blot: Myosin light chain 3 Antibody (NOQ7.5.4D) [NB120-11083] - Whole cell extract of rabbit tongue was separated on SDS-PAGE. Lane A was blotted with NB120-11083 (1:5,000), and Lane B was the Negative control ...read more
Immunohistochemistry: Myosin light chain 3 Antibody (NOQ7.5.4D) [NB120-11083] - Staining of formalin-fixed, paraffin-embedded section of rabbit tongue using NB120-11083 (1:4000). Note the specific staining of slow ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB120-11083
    • Availability
      Product Discontinued

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Myosin Skeletal Antibody (NOQ7.5.4D) Summary

Immunogen
Human skeletal muscle myosin purified from myofibrils.
Epitope
Monoclonal Anti Myosin (Skeletal, Slow) recognizes an epitope located on the heavy meromyosin portion of human adult skeletal muscle slow myosin.
Localization
Cytoplasmic
Marker
Slow Myosin, Heavy Chain Marker
Specificity
Reacts with human adult skeletal muscle slow myosin. Does not react with the fast myosin heavy chain nor with embryonic or neonatal rat skeletal myosins, nor with cat fetal/embryonic skeletal myosin. Also reacts with human adult type I skeletal muscle fibers and with cardiac muscle cells. No reactivity detected with human or rat adult smooth muscle or with non-muscle tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MYH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot Assay Dependent
  • Electron Microscopy
  • ELISA Assay Dependent
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:4000
  • Radioimmunoassay
Application Notes
Monoclonal Anti-Myosin (Skeletal, Slow) may be used for the localization of slow (Type I) myosin. It may be used in immunoblotting, ELISA, solid-phase RIA, immunohistology (frozen, formalin-fixed, paraffin-embedded and methacarn-fixed paraffin-embedded tissue sections) and immunoelectronmicroscopy. Detection of slow myosin in formalin-fixed, paraffin-embedded, tissues is greatly enhanced by proteolytic digestion of the preparation.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat, Cow, Cat, Chicken, Dog, Goat, Guinea pig, Pig, Rabbit and Sheep. This product may react with Hamster. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Myosin Skeletal Antibody (NOQ7.5.4D)

  • MGC133384
  • MYH1
  • MYHa
  • MyHC-2x
  • MyHC-2X/D
  • myHC-IIx/d
  • MYHSA1
  • Myosin heavy chain 1
  • Myosin heavy chain 2x
  • Myosin heavy chain IIx/d
  • Myosin heavy chain, skeletal muscle, adult 1
  • myosin, heavy chain 1, skeletal muscle, adult
  • myosin, heavy polypeptide 1, skeletal muscle, adult
  • myosin-1

Background

Myosin is a 500 kD protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200 kD each) and four light chains (15-20 kD each). Myosin molecules consist of two major regions; the tail (rod) and head. These molecules aggregate into filaments through the tail region and interact with actin and ATP through the head region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Myosin Skeletal Antibody (NB120-11083). (Showing 1 - 1 of 1 FAQ).

  1. Our customer found NB120-11083 may be suitable for her test. However, it is the frozen section which she would like to perform IHC on. So the customer would like to know if it is possible to perform IHC with frozen sections with this antibody.
    • We haven't validated this Ab for use with frozen sections for IHC however your customer could test this as part of our Innovators Reward Program. It is likely to work fine as it has been validated for use in IHC-P.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYH1
Entrez
Uniprot