Myosin light chain kinase Overexpression Lysate (Denatured)

Images

 
Lane 1: MYLK transfected lysate (109.23 KDa) Lane 2: Non-transfected lysate.
MYLK transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Myosin light chain kinase Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-MYLK full-length
Gene
MYLK

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for Myosin light chain kinase

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-MYLK antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Myosin light chain kinase Overexpression Lysate (Denatured)

  • DKFZp686I10125
  • EC 2.7.11
  • EC 2.7.11.18
  • FLJ12216
  • Kinase-related protein
  • KRP
  • light polypeptide kinase
  • MLCK1
  • MLCK108
  • MLCKMLCK210
  • MSTP083
  • MYLK1
  • myosin light chain kinase
  • smooth muscle myosin light chain kinase
  • smooth muscle
  • telokin

Background

This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MYLK