Myosin Heavy Chain Antibody (MY32)

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, GP, RbSpecies Glossary
Applications IHC, IHC-P
Clone
MY32
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Myosin Heavy Chain Antibody (MY32) Summary

Immunogen
Rabbit muscle myosin.
Localization
Cytoplasmic
Specificity
This antibody recognizes the heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. Rather, it stains the fast twitch (type II) isomyosin molecule. This antibody cross-reacts with myosin from rabbit, rat, mouse, bovine, chicken, and guinea pig.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MYH14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
Application Notes
This antibody has been pretitered and quality controlled to work on formalin-fixed, paraffin-embedded and acetone fixed cryostat tissue sections. No further titration is required.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
No buffer
Preservative
No Preservative
Purity
Protein A or G purified

Notes

This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Alternate Names for Myosin Heavy Chain Antibody (MY32)

  • DFNA4
  • FLJ13881
  • FLJ43092
  • KIAA2034DKFZp667A1311
  • MHC16
  • MYH14 variant protein
  • MYH17
  • MYHC
  • Myosin heavy chain 14
  • Myosin Heavy Chain
  • Myosin heavy chain, non-muscle IIc
  • myosin
  • myosin, heavy chain 14
  • myosin, heavy chain 14, non-muscle
  • myosin, heavy polypeptide 14
  • myosin-14
  • NMHC II-C
  • NMHC-II-C
  • Non-muscle myosin heavy chain IIc
  • nonmuscle myosin heavy chain II-C

Background

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MYH14