Myogenin Antibody (F5D) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with Myogenin MAb (F5D)
Flow Cytometry: Myogenin Antibody (F5D) - Azide and BSA Free [NBP2-34616] - An intracellular stain was performed on RH-30 cells with Myogenin Antibody [F5D] NBP2-34616PCP (blue) and a matched isotype control (orange). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, FeSpecies Glossary
Applications ELISA, IHC, IHC-P, CyTOF-ready
Clone
F5D
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Myogenin Antibody (F5D) - Azide and BSA Free Summary

Immunogen
Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)
Localization
Nuclear
Marker
Skeletal Muscle Marker
Specificity
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1-5ug/ml for coating
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • CyTOF-ready
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Alternate Names for Myogenin Antibody (F5D) - Azide and BSA Free

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34616AF350
Alexa Fluor 405 NBP2-34616AF405
Alexa Fluor 488 NBP2-34616AF488
Alexa Fluor 532 NBP2-34616AF532
Alexa Fluor 594 NBP2-34616AF594
Alexa Fluor 647 NBP2-34616AF647
Alexa Fluor 700 NBP2-34616AF700
Alexa Fluor 750 NBP2-34616AF750
Biotin NBP2-34616B
DyLight 350 NBP2-34616UV
DyLight 405 NBP2-34616V
DyLight 488 NBP2-34616G
DyLight 550 NBP2-34616R
DyLight 594 NBP2-34616DL594
DyLight 650 NBP2-34616C
DyLight 680 NBP2-34616FR
DyLight 755 NBP2-34616IR
FITC NBP2-34616F
HRP NBP2-34616H
Janelia Fluor 549 NBP2-34616JF549
Janelia Fluor 646 NBP2-34616JF646

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Bioinformatics

Gene Symbol MYOG