MyoD Antibody (5.8A) [DyLight 405LS]



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MyoD Antibody (5.8A) [DyLight 405LS] Summary

The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
In Rh-30, a ~45 kDa band should be observed.
IgG1 Kappa
Protein G purified
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  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
There is considerable literature published using the MyoD, Clone 5.8 antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MyoD Antibody (5.8A) [DyLight 405LS]

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NB100-56511B
DyLight 350 Labeled NB100-56511UV
DyLight 405 Labeled NB100-56511V
DyLight 488 Labeled NB100-56511G
DyLight 550 Labeled NB100-56511R
DyLight 650 Labeled NB100-56511C
DyLight 680 Labeled NB100-56511FR
DyLight 755 Labeled NB100-56511IR
HRP Labeled NB100-56511H

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Gene Symbol MYOD1