MyD88 Antibody


Western Blot: MyD88 Antibody [NB300-946] - (0.03ug/ml) of human thymus lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: MyD88 Antibody [NB300-946] - (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained more
Sandwich ELISA: MyD88 Antibody [NB300-946] - (5ug/ml) as the reporter with capture rabbit antibody (5ug/ml).

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MyD88 Antibody Summary

Peptide with sequence C-IKYKAMKKEFP corresponding to internal region according to NP_002459.1.
Immunogen affinity purified
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  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Sandwich ELISA 5 - 10 ug/ml
Application Notes
EIA: Sandwich ELISA with increasing amount of recombinant MYD88 captured by a rabbit antibody. WB: Approx. 33 kDa band observed in human thymus lysates (calculated MW of 33.2 kDa band according to NP_002459.1). IHC-P: Human tonsil shows staining of clusters of cells some are likely plasma cells.
Positive Control
MyD88 Lysate (NBL1-13419)
MyD88 Lysate (NBP2-65269)
Read Publication using NB300-946.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for MyD88 Antibody

  • MyD88
  • MYD88D
  • myeloid differentiation primary response gene (88)
  • myeloid differentiation primary response protein MyD88


Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL1R and is involved in the inflammatory response induced by IL1, IL18 and LPS. MyD88 associates with and recruits IRAK to IL1 receptor complex in response to IL1. This pathway further leads to activation of NFkB. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL1 and IL18, and MyD88 deficient mice lack responses to bacterial product LPS that employs Toll like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 has a wide tissue distribution.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MyD88 Antibody (NB300-946)(1)

Showing Publication 1 - 1 of 1.
Publication using NB300-946 Applications Species
Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med 2005 Jan 21 [PMID: 15662540]

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on MyD88.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

MYD88 Expression and Tumorigenesis
MyD88, also called myeloid differentiation primary response gene 88, encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. The innate immune system recognizes the presence of bacterial pathogens thro...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

MYD88: Fanning Inflammation and Immune Responses
Myeloid differentiation primary response gene 88 (MYD88) encodes a cytosolic adapter protein that plays an essential role in innate and adaptive immune responses. MYD88 protein functions as an essential signal transducer in the interleukin-1 and in To...  Read full blog post.

MyD88 Antibodies for IL Signaling and Immunity Research
The myeloid differentiation protein MyD88 (myeloid differentiation primary response protein) was originally identified and characterized as a primary upregulated response gene in interleukin-6 mediated myeloid differentiation. Now, MyD88 is known to b...  Read full blog post.

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Gene Symbol MYD88