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Muscleblind-like 1 Antibody (HL-1822 3A4-1E9)

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Western Blot: Muscleblind-like 1 Antibody (HL-1822 3A4-1E9) [NB110-37256] - Detection of Muscleblind in HeLa whole cell extract (33ug) using NB 110-37256.
Western Blot: Muscleblind-like 1 Antibody (HL-1822 3A4-1E9) [NB110-37256] - Detection of Muscleblind in HeLa whole cell extract (33ug) using NB 110-37256.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
HL-1822 3A4-1E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Muscleblind-like 1 Antibody (HL-1822 3A4-1E9) Summary

Immunogen
Recombinant human Muscleblind-like 1 protein. [UniProt# Q9NR56]
Epitope
The epitope recognized is between amino acids 116-182.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MBNL1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This antibody is useful for Western blot where a band is seen at ~40 kDa, Immunofluorescence and Immunohistochemistry on paraffin-embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 4
using
NB110-37256 in the following applications:
Publications
Read Publication using
NB110-37256 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
10mM Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Muscleblind-like 1 Antibody (HL-1822 3A4-1E9)

  • DKFZp686P06174
  • EXP40
  • EXP42
  • EXPKIAA0428EXP35
  • MBNL
  • muscleblind (Drosophila)-like
  • muscleblind-like (Drosophila)
  • muscleblind-like protein 1
  • Triplet-expansion RNA-binding protein

Background

pre-mRNAs splicing /removal of non-coding regions is a critical process which contributes to a diverse proteome as well as gene expression regulation, and there are several splicing factors that are exclusively involved in a subset of splicing decisions - human muscleblind-like family of RNA-binding proteins: MBNL1/2/3 also known as MBNL/EXP, MBLL/MPL1, and MBXL/CHCR, respectively. Muscleblind-like 1 (MBNL1) is a known mediator of pre-mRNA alternative splicing regulation and it acts either as activator or repressor of splicing on specific pre-mRNA targets. Several pathologies are linked to expansion of polynucleotide sequences (mostly trinucleotide repeats) and CUG/CCUG expansion RNAs accumulates in Myotonic Dystrophy patient cells wherein it contributes to the disease by dysregulating alternative splicing factors such as CUGBP, Elav-like Family Member 1 (CELF1) and MBNL1. The expanded CUG repeats sequester MBNL proteins into nuclear foci, leading to loss of active protein and the sequestration of MBNL1 leads to missplicing of developmentally regulated events that are responsible for symptoms in DM types 1 and 2, such as the missplicing of the chloride channel (CLCN1) leading to myotonia. CELF1 and MBNL1 regulate a number of common splicing events, but in opposite directions and loss of MBNL1 activity is more critical than upregulation of CELF1 activity for abnormal splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Muscleblind-like 1 Antibody (NB110-37256)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Review for Muscleblind-like 1 Antibody (NB110-37256) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-37256:
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Western Blot Muscleblind-like 1 NB110-37256
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reviewed by:
Anonymous
 WB Human 01/24/2016
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Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
Lot012404

Blocking

Blocking Details5% Milk, 1% BSA in TBS-T, 1H room temperature

Primary Anitbody

Dilution Ratio1:1000 in 5% Milk, 1% BSA in TBS-T, 1H room temperature

Secondary Antibody

Secondary DescriptionAmersham ECL mouse IgG, HRP-linked whole Ab (from sheep)
Secondary Manufacturer Cat#NA931-1ML
Secondary Concentration1:5000

Details

Detection NotesECL, 5 minute exposure

Comments

Comments40ug of whole cell lysate loaded.

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Positive Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Western Blot Muscleblind-like 1 NB110-37256
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 Anonymous
01/24/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol MBNL1