This antibody is useful for Western blot where a band is seen at ~40 kDa, Immunofluorescence and Immunohistochemistry on paraffin-embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
Tissue culture supernatant
Alternate Names for Muscleblind-like 1 Antibody (HL-1822 3A4-1E9)
muscleblind-like protein 1
Triplet-expansion RNA-binding protein
pre-mRNAs splicing /removal of non-coding regions is a critical process which contributes to a diverse proteome as well as gene expression regulation, and there are several splicing factors that are exclusively involved in a subset of splicing decisions - human muscleblind-like family of RNA-binding proteins: MBNL1/2/3 also known as MBNL/EXP, MBLL/MPL1, and MBXL/CHCR, respectively. Muscleblind-like 1 (MBNL1) is a known mediator of pre-mRNA alternative splicing regulation and it acts either as activator or repressor of splicing on specific pre-mRNA targets. Several pathologies are linked to expansion of polynucleotide sequences (mostly trinucleotide repeats) and CUG/CCUG expansion RNAs accumulates in Myotonic Dystrophy patient cells wherein it contributes to the disease by dysregulating alternative splicing factors such as CUGBP, Elav-like Family Member 1 (CELF1) and MBNL1. The expanded CUG repeats sequester MBNL proteins into nuclear foci, leading to loss of active protein and the sequestration of MBNL1 leads to missplicing of developmentally regulated events that are responsible for symptoms in DM types 1 and 2, such as the missplicing of the chloride channel (CLCN1) leading to myotonia. CELF1 and MBNL1 regulate a number of common splicing events, but in opposite directions and loss of MBNL1 activity is more critical than upregulation of CELF1 activity for abnormal splicing.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.