Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [HRP]

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Order Details


    • Catalog Number
      NB120-20563
    • Availability
      Product Discontinued

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Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Fructose-6-Phosphate Kinase [Rabbit Muscle]
Localization
Cytoplasmic
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Goat
Gene
PFKM
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000
Readout System

Reactivity Notes

Rabbit

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
Gentamicin Sulfate
Purity
Ion exchange chromatography

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [HRP]

  • 6-phosphofructokinase, muscle type
  • EC 2.7.1,6-phosphofructo-1-kinase
  • EC 2.7.1.11
  • GSD7
  • MGC8699
  • Muscle Phosphofructokinase
  • PFK1
  • PFK-1
  • PFKA
  • PFK-A
  • PFKM
  • PFKX
  • Phosphofructo-1-kinase isozyme A
  • Phosphofructokinase 1
  • phosphofructokinase, muscle
  • phosphofructokinase, polypeptide X
  • Phosphofructokinase-1
  • Phosphofructokinase-M
  • Phosphohexokinase

Background

Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PFKM
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