Mucin 5AC Antibody (SPM297) [Allophycocyanin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Mk, Rb, Bv(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM297
Clonality
Monoclonal
Host
Mouse

Mucin 5AC Antibody (SPM297) [Allophycocyanin] Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Allophycocyanin
Format
Allophycocyanin (A=620-650, E=660-670)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Reactivity Notes

Expected cross reactivity based on sequence identity: Hedgehog

Alternate Names for Mucin 5AC Antibody (SPM297) [Allophycocyanin]

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mucin 5AC Antibody (NBP2-34758APC) (0)

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MUC5AC

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