Mucin 5AC Antibody (45M1) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) - Azide and BSA Free [NBP2-32732] - Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1).
Flow Cytometry: Mucin 5AC Antibody (45M1) [Azide Free] [NBP2-32732] - Analysis of fixed (70% ethanol or 80% methanol) and permeabilized A549 cells
Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) [Azide Free] [NBP2-32732] - Human stomach.
Immunohistochemistry-Paraffin: Mucin 5AC Antibody (45M1) - Azide and BSA Free [NBP2-32732] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
45M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Mucin 5AC Antibody (45M1) - Azide and BSA Free Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1.0ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
  • CyTOF-ready

Reactivity Notes

Hedgehog

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Mucin 5AC Antibody (45M1) - Azide and BSA Free

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Background

MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. Mucus protects the tissue surface from mechanical damage, stabilizes the luminal microenvironment, and traps pathogens including bacteria and viruses for mucociliarly clearance. MUC5AC, like other family members, is differentially expressed. MUC5AC is expressed in airway and gastric epithelial cells and highly expressed in colorectal carcinomas. Although not normally detected by antibody in adult colon, MUC5AC is detectable in fetal colon. It is thought that positive MUC5AC antibody staining in colorectal cancer may be due to the resurgence of MUC5AC expression or the unmasking of an embryonic MUC5AC antibody reactive epitope during tumorigenesis. There are qualitative and quantitative alterations of MUC5AC expression in association with various pathological conditions. For example, S dysentariae infection induced MUC5AC expression in HT29 cells which was inhibited by polymyxin B pretreatment (Raja et al, 2011). Antibody studies identified elevated MUC5AC levels in benign and malignant gallbladder lesions (Xiong et al, 2011). On the other hand, MUC5AC expression is decreased or has abnormal glycosylation in the goblet cells of conjunctiva in a number of eye inflammatory conditions including Sjogren, Steven-Johnson and dry eye syndromes (Contreras-Ruiz, 2012). The MUC5AC clone 45M1 antibody is widely used. For example, it has been used as part of an antibody panel to help distinguish esophageal adenocarcinoma (MUC5AC/+) from squamous cell carcinoma (MUC5AC/-) (DiMaio et al, 2012)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MUC5AC