Mucin 5AC Antibody (45M1) - Azide and BSA Free Summary
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Protein G purified
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- Western Blot 0.5-1.0ug/ml
- ELISA 1-5ug/ml for coating
- Flow Cytometry 0.5-1.0ug/million cells
- Immunocytochemistry/Immunofluorescence 1-2ug/ml
- Immunohistochemistry-Frozen 0.5ug/ml
- Immunohistochemistry-Paraffin 0.5-1.0ug/ml
- Immunoprecipitation 1-2ug/500ug protein lysate
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM PBS - BSA free
Protein G purified
Alternate Names for Mucin 5AC Antibody (45M1) - Azide and BSA Free
- gastric mucin
- lewis B blood group antigen
- major airway glycoprotein
- mucin 5, subtypes A and C, tracheobronchial/gastric
- mucin 5AC, oligomeric mucus/gel-forming pseudogene
- mucin 5AC, oligomeric mucus/gel-forming
- mucin-5 subtype AC, tracheobronchial
- tracheobronchial mucin
MUC5AC (gastric mucin) belongs to the gel-forming mucin family of glycoproteins, the major components of the protective mucus layer on the mucosal surfaces of epithelial tissues. Mucus protects the tissue surface from mechanical damage, stabilizes the luminal microenvironment, and traps pathogens including bacteria and viruses for mucociliarly clearance. MUC5AC, like other family members, is differentially expressed. MUC5AC is expressed in airway and gastric epithelial cells and highly expressed in colorectal carcinomas. Although not normally detected by antibody in adult colon, MUC5AC is detectable in fetal colon. It is thought that positive MUC5AC antibody staining in colorectal cancer may be due to the resurgence of MUC5AC expression or the unmasking of an embryonic MUC5AC antibody reactive epitope during tumorigenesis. There are qualitative and quantitative alterations of MUC5AC expression in association with various pathological conditions. For example, S dysentariae infection induced MUC5AC expression in HT29 cells which was inhibited by polymyxin B pretreatment (Raja et al, 2011). Antibody studies identified elevated MUC5AC levels in benign and malignant gallbladder lesions (Xiong et al, 2011). On the other hand, MUC5AC expression is decreased or has abnormal glycosylation in the goblet cells of conjunctiva in a number of eye inflammatory conditions including Sjogren, Steven-Johnson and dry eye syndromes (Contreras-Ruiz, 2012). The MUC5AC clone 45M1 antibody is widely used. For example, it has been used as part of an antibody panel to help distinguish esophageal adenocarcinoma (MUC5AC/+) from squamous cell carcinoma (MUC5AC/-) (DiMaio et al, 2012)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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