Mucin 5AC Antibody (2-12M1)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
2-12M1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Mucin 5AC Antibody (2-12M1) Summary

Immunogen
The antibody reacts with the MUC5AC antigen.
Specificity
2-12M1 reacts with peptide core of gastric mucin (MUC5AC). Source: A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification). Splenocytes were fused with mouse myeloma SP2/0 cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemistry on cultured cells: strongly positive on ethanol fixed epithelial cells. Immunohistochemistry on frozen ethanol fixed section. Immunohistochemistry on paraffin section: very strong positive reaction with ethanol fixed tissues. On forma

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg/ml PBS
Preservative
0.05% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Mucin 5AC Antibody (2-12M1)

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Background

Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric mucin M1 antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden M1 antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MUC5AC