MUC1 Antibody (VU-5F12)

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Product Details

Summary
Product Discontinued
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    • Catalog Number
      NBP1-42153
    • Availability
      Product Discontinued

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MUC1 Antibody (VU-5F12) Summary

Immunogen
The antibody reacts with the MUC1 antigen.
Specificity
VU-5F12 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largly consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of VU-5F12 is as established with “epitope fingerprinting”. Source: A Balb/c mouse was immunized with a MUC1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH conjugated to bovin serum albumin. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

Reactivity Notes

Human,Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg/ml PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for MUC1 Antibody (VU-5F12)

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Background

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for MUC1 Antibody (NBP1-42153). (Showing 1 - 1 of 1 FAQ).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

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Bioinformatics

Gene Symbol MUC1
Entrez