MUC-4 Antibody

Images

 
Western Blot: MUC-4 Antibody [NBP1-52193] - Analysis of MUC4 beta in A. MCF7 whole cell extract and B. HeLa whole cell extract.
Immunocytochemistry/ Immunofluorescence: MUC-4 Antibody [NBP1-52193] - ICC staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MUC-4 Antibody [NBP1-52193] - IHC staining of MUC4 in mouse prostate
Simple Western: MUC-4 Antibody [NBP1-52193] - Simple Western lane view shows a specific band for MUC4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.56 mg/ml

Order Details

MUC-4 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human MUC4 protein (within residues 1950-2100). [Swiss-Prot Q99102]
Localization
MUC4 beta chain: Cell membrane; Single-pass membrane protein.
Specificity
This antibody is specific for the beta chain of MUC4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MUC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This MUC4 antibody is useful for IHC, ICC/IF, and Western blot where bands are seen ~78 kDa representing MUC4 beta and ~50 kDa. The identity of the 50 kDa band is unknown. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Does not work for IP as per customer feedback.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC1)
MCF-7 Lysate (NB820-59465)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-52193 in the following applications:

Publications
Read Publication using
NBP1-52193 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.56 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MUC-4 Antibody

  • Ascites sialoglycoprotein
  • ASGP
  • HSA276359
  • MUC4
  • MUC-4
  • mucin 4, cell surface associated
  • mucin 4, tracheobronchial
  • mucin-4
  • Pancreatic adenocarcinoma mucin
  • Testis mucin
  • Tracheobronchial mucin

Background

MUC4 (Mucin-4) is a large transmembrane protein that belongs to mucins family which contains high-molecular weight glycoproteins with oligosaccharides attached to serine or threonine residues of mucin core protein backbone by O-glycosidic linkages. Mucins have two catagories: transmembrane mucins (MUC1, MUC3, MUC4, MUC12, MUC13, MUC15, MUC16, MUC17, MUC20 and MUC21) and secreted mucins (MUC2, MUC5AC, MUC5B, MUC6, MUC7, and MUC19). Mucins are produced by epithelial cells and serve protective as well as lubricating role, however, in damaged epithelia or tumor cells, there is a loss of polarity that occurs in association with activation of proliferation/survival mechanisms. MUC4 is normally expressed in many epithelial tissues such as respiratory, colonic, and vaginal epithelia, and its expression is altered in various tumors including fibromyoxoid sarcoma and carcinomas of pancreas, bile duct, pancreatic duct etc. MUC4's mediated activation of ErbB2 downstream signaling may lead to cell proliferation/survival, and tumor cells can exploit versatile anti-apoptotic activities of MUC4 for augmenting survival or developing resistance to therapeutic agents. MUC4 has also been shown to enhance invasion and metastasis in prostate cancer, partly through regulating the EMT process (N-cadherin upregulation) and stabilizing FGFR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-22711
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-11197
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
NBP2-15196
Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC
NBP1-92151
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-44374
Species: Hu
Applications: Flow, IHC-P, IF
NBP2-44432
Species: Hu
Applications: Flow, IHC-P, PAGE, IF
NBP1-82045
Species: Hu
Applications: IHC, IHC-P
NB600-1468
Species: Hu
Applications: WB, IHC-P, S-ELISA
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB200-124
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, RIA
MAB3481
Species: Hu
Applications: Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-24566
Species: Hu
Applications: WB, IHC-P
NBP1-52193
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for MUC-4 Antibody (NBP1-52193)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for MUC-4 Antibody (NBP1-52193) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-52193:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MUC-4 NBP1-52193
Enlarge
reviewed by:
Anonymous
WB Mouse 01/24/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse lung homogenate
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC-4 Antibody (NBP1-52193) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MUC-4 Products

Bioinformatics Tool for MUC-4 Antibody (NBP1-52193)

Discover related pathways, diseases and genes to MUC-4 Antibody (NBP1-52193). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MUC-4 Antibody (NBP1-52193)

Discover more about diseases related to MUC-4 Antibody (NBP1-52193).
 

Pathways for MUC-4 Antibody (NBP1-52193)

View related products by pathway.

PTMs for MUC-4 Antibody (NBP1-52193)

Learn more about PTMs related to MUC-4 Antibody (NBP1-52193).
 

Research Areas for MUC-4 Antibody (NBP1-52193)

Find related products by research area.

Blogs on MUC-4

There are no specific blogs for MUC-4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
01/24/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MUC4
Entrez
Uniprot