MUC-4 Antibody


Western Blot: MUC-4 Antibody [NBP1-52193] - Analysis of MUC4 beta in A. MCF7 whole cell extract and B. HeLa whole cell extract.
Immunocytochemistry/ Immunofluorescence: MUC-4 Antibody [NBP1-52193] - ICC staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MUC-4 Antibody [NBP1-52193] - IHC staining of MUC4 in mouse prostate
Simple Western: MUC-4 Antibody [NBP1-52193] - Simple Western lane view shows a specific band for MUC4 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
0.56 mg/ml

Order Details

MUC-4 Antibody Summary

A genomic peptide made to an internal region of the human MUC4 protein (within residues 1950-2100). [Swiss-Prot Q99102]
MUC4 beta chain: Cell membrane; Single-pass membrane protein.
This antibody is specific for the beta chain of MUC4.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.1% BSA, and 50% Glycerol
0.05% Sodium Azide
0.56 mg/ml
Immunogen affinity purified


  • Western Blot 1:5000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This MUC4 antibody is useful for IHC, ICC/IF, and Western blot where bands are seen ~78 kDa representing MUC4 beta and ~50 kDa. The identity of the 50 kDa band is unknown. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Does not work for IP as per customer feedback. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

Human and mouse.


Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MUC-4 Antibody

  • Ascites sialoglycoprotein
  • ASGP
  • HSA276359
  • MUC4
  • MUC-4
  • mucin 4, cell surface associated
  • mucin 4, tracheobronchial
  • mucin-4
  • Pancreatic adenocarcinoma mucin
  • Testis mucin
  • Tracheobronchial mucin


MUC4 (Mucin-4) is a large transmembrane protein that belongs to mucins family which contains high-molecular weight glycoproteins with oligosaccharides attached to serine or threonine residues of mucin core protein backbone by O-glycosidic linkages. Mucins have two catagories: transmembrane mucins (MUC1, MUC3, MUC4, MUC12, MUC13, MUC15, MUC16, MUC17, MUC20 and MUC21) and secreted mucins (MUC2, MUC5AC, MUC5B, MUC6, MUC7, and MUC19). Mucins are produced by epithelial cells and serve protective as well as lubricating role, however, in damaged epithelia or tumor cells, there is a loss of polarity that occurs in association with activation of proliferation/survival mechanisms. MUC4 is normally expressed in many epithelial tissues such as respiratory, colonic, and vaginal epithelia, and its expression is altered in various tumors including fibromyoxoid sarcoma and carcinomas of pancreas, bile duct, pancreatic duct etc. MUC4's mediated activation of ErbB2 downstream signaling may lead to cell proliferation/survival, and tumor cells can exploit versatile anti-apoptotic activities of MUC4 for augmenting survival or developing resistance to therapeutic agents. MUC4 has also been shown to enhance invasion and metastasis in prostate cancer, partly through regulating the EMT process (N-cadherin upregulation) and stabilizing FGFR1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC-4 Antibody (NBP1-52193) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol MUC4

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