Recombinant Human MUC-1 Protein Summary
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 315-420 of Human MUC-1
Source: Wheat Germ
Amino Acid Sequence: NSSLEDPSTDYYQELQRDISEMFLQIYKQGGFLGLSNIKFRPGSVVVQLTLAFREGTINVHDMETQFNQYKTEAASRYNLTISDVSVSDVPFPFSAQSGAGVPGWG
|Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
| Protein/Peptide Type
Partial Recombinant Protein
>80% by SDS-PAGE and Coomassie blue staining
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
>80% by SDS-PAGE and Coomassie blue staining
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Recombinant Human MUC-1 Protein
- Breast carcinoma-associated antigen DF3
- Carcinoma-associated mucin
- CD227 antigen
- DF3 antigen
- H23 antigen
- mucin 1, cell surface associated
- mucin 1, transmembrane
- Peanut-reactive urinary mucin
- Polymorphic epithelial mucin
- tumor associated epithelial mucin
- Tumor-associated epithelial membrane antigen
- Tumor-associated mucin
This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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Publications for MUC-1 Partial Recombinant Protein (H00004582-Q01)(3)
Showing Publications 1 - 3
|Publications using H00004582-Q01
| Lu H, Ladd J, Feng Z et al. Evaluation of Known Oncoantibodies, HER2, p53, and Cyclin B1, in Prediagnostic Breast Cancer Sera. Cancer Prev Res (Phila). 2012 Jul 03 [PMID: 22715141]
| Florea A, Taleat Z, Cristea C et al. Label free MUC1 aptasensors based on electrodeposition of gold nanoparticles on screen printed electrodes. Electrochemistry Communications. 2013 Aug
| Desmetz C, Bascoul-Mollevi C, Rochaix P et al. Identification of a New Panel of Serum Autoantibodies Associated with the Presence of In situ Carcinoma of the Breast in Younger Women. Clin Cancer Res 15(14):4733-41. 2009 Jul 15. [PMID: 19584157]
Reviews for MUC-1 Partial Recombinant Protein (H00004582-Q01) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for MUC-1 Partial Recombinant Protein (H00004582-Q01). (Showing 1 - 3 of 3 FAQs).
I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
- Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.
I am interested in purchasing MUC1 protein from you and it would be a great help if you could answer a few of my questions. 1) How much of the glycoprotein is glycosylated? 2) Is it soluble in water? 3) It says on the website that the product is not active, what exactly do you mean by that?
- This protein is a recombinant protein. It does not undergo glycosylation. It may not be folded in the same way as the native protein therefore it may be not act in the same way. This should only be used for WB and as a positive control in ELISA. It is soluble in water.
Could you recommend us a protocol for ELISA test in a sandwich format for MUC1 protein catalog # H00004582-Q01 using catalog # NB100-65647 as primary antibody and catalog # NBP1-61561 as secondary with a third labeled antibody? Regarding the request below with that pair of antibodies and that recombinant protein: could you help us with another primary monoclonal antibody to replace the NB100-65647 antibody with it, so we could use it in a sandwich assay with the protein H00004582-Q01 and the NBP1-61561. Thank you. Also do you know if this recombinant protein is tumoral MUC1 or normal MUC1, because they have different structures.
- Unfortunately, the antibody and protein combination you have chosen will not work. As is stated in our datasheet for NB100-65647 The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP. Unfortunately, this epitope is not present in the partial recombinant protein H00004582-Q01. While NB100-65647 and NBP1-61561 are suitable pairs to use for ELISA, H00004582-Q01 is not a suitable standard for use with this pair, and unfortunately it does not look like we carry a suitable standard for you. I apologize for the inconvenience. Please see our ELISA protocol. H00004582-Q01 is produced by a Taiwanese company called Abnova and we distribute it for them. It is produced in their cell-free wheat germ expression system. I would recommend using one of Abnova's matched antibody pairs for use in ELISA if you are going to use H00004582-Q01 as a standard. I would recommend using the H00004582-AP41 antibody pair.
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