MUC-1 Antibody

Images

 
Immunohistochemistry-Paraffin: MUC-1 Antibody [NB120-15481] - MUC1 in mouse uterus. Image from verfied customer review.
Immunohistochemistry-Paraffin: MUC-1 Antibody [NB120-15481] - IHC analysis of MUC1 in paraffin embedded mouse liver tissue. Image courtesy of anonymous customer product review.
Immunohistochemistry-Paraffin: MUC-1 Antibody [NB120-15481] - Breast carcinoma stained with Anti-Muc-1 antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

MUC-1 Antibody Summary

Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail of human MUC-1.
Localization
Cell Membrane and Cytoplasmic
Specificity
MUC1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MUC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining. Predicted molecular weight: 122 kDa. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26002932)
Theoretical MW
350 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
MCF-7 Lysate (NB820-59465)
MCF-7 Lysate (NB820-59465)
Reviewed Applications
Read 2 Reviews rated 5
using
NB120-15481 in the following applications:

Publications
Read Publications using
NB120-15481 in the following applications:

Reactivity Notes

Predicted by homology: Bovine, Rabbit, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MUC-1 Antibody

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-11197
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
NBP2-46231
Species: Hu
Applications: WB, IHC
AF4478
Species: Hu
Applications: WB, IHC
H00005670-T01
Species: Hu
NBP2-15196
Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP1-52193
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00054474-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-44374
Species: Hu
Applications: Flow, IHC-P, IF
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
NB120-15481
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P

Publications for MUC-1 Antibody (NB120-15481)(4)

Reviews for MUC-1 Antibody (NB120-15481) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 1 application: IHC-P.

Reviews using NB120-15481:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MUC-1 NB120-15481
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 05/05/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
SpeciesMouse
Lot90900
Pub Med ID21330545
FileView PDF
Immunohistochemistry-Paraffin MUC-1 NB120-15481
Enlarge
reviewed by:
Marion Pollheimer
IHC-P Mouse 09/15/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Liver Tissue
SpeciesMouse
CommentsSample: Mouse tissue (liver)Fixation: FormalinHeat mediated - Buffer/Enzyme Used: 10mM citric acid buffer Dilution: 1/100 Incubation time: 18 hours, Temperature: 4°C

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 18 hours, Primary Ab Incubation Temp: 4 degrees Celsius

Details

Detection NotesDetection Method: AEC, Antigen Retrieval: 10mM citric acid buffer, 40 minutes at 98 degrees Celsius
Fixation DetailsSample Preparation: PFA fixed and paraffin embedded

Comments

CommentsSample: Mouse tissue (liver)Fixation: FormalinHeat mediated - Buffer/Enzyme Used: 10mM citric acid buffer Dilution: 1/100 Incubation time: 18 hours, Temperature: 4°C

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for MUC-1 Antibody (NB120-15481). (Showing 1 - 2 of 2 FAQs).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.
  2. I have a customer who is asking about whether MUC1 Partial Recombinant Protein from Novus (Cat# H00004582-Q01) can be used with MUC1 antibody (Cat# NB120-15481). Their question was  "Does the MUC1 antibody NB120-15481 have a high affinity for H00004582-Q01, given that it is recombinant. I need to find the protein with higher capacity for binding to that antibody." According to the product datasheet for the has been raised against a "Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail of human MUC-1". The partial recombinant MUC1 protein is made of "MUC1 (NP_877418.1, 315 a.a. - 420 a.a.)". So it depends if the MUC1 sequence that the MUC1 antibody was raised against is within this partial recombinant MUC1 protein sequence. The precise amino acid sequence of the immunogen for MUC1 antibody is not listed on the datasheet due to commercial sensitivity. Can you please advise if the amino acid sequence of the MUC1 antibody immunogen is within the partial recombinant MUC1 protein? If so, do you predict the antibody will bind this recombinant protein with high affinity? If not, is there an alternative product you can suggest? Just an additional note that the customer has just clarified: The products are to be used for "developing immunoassays with biosensors so essentially I need this antibody-protein pair for optimization of detection conditions before trying detection in real samples.
    • Our MUC1 antibody with catalogue number NB120-15481 was raised to a synthetic peptide derived from the cytoplasmic tail of human MUC1. According to the Uniprot listing (P15941) the cytoplasmic region of MUC1 is from amino acids 1182-1255. Our partial recombinant MUC1 protein with catalogue number H00004582-Q01 is derived from amino acids 315-420 so it is unlikely that NB120-15481 would recognise this protein. Unfortunately this partial recombinant protein is the only human MUC1 protein that we sell. This protein is produced by Abnova, for whom we distribute, but having checked their website I see that they do not stock any other MUC1 proteins either. Our MUC1 antibody with catalogue number NB300-708 was raised to a synthetic peptide representing a 20 amino acid repeat sequence from Mucin 1. A 60 amino acid long peptide was conjugated to KLH and was used to immunize rabbits. The 20 amino acid repeat sequence used was VTSAPDTRPAPGSTAPPAHG. This repeat sequence is present from amino acids 61-1020, and so it is entirely possible that NB300-708 will detect H00004582-Q01. We have not ourselves tested the binding of NB300-708 to the partial recombinant protein. But this may be a good option for your customer to try.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MUC-1 Products

Bioinformatics Tool for MUC-1 Antibody (NB120-15481)

Discover related pathways, diseases and genes to MUC-1 Antibody (NB120-15481). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MUC-1 Antibody (NB120-15481)

Discover more about diseases related to MUC-1 Antibody (NB120-15481).
 

Pathways for MUC-1 Antibody (NB120-15481)

View related products by pathway.

PTMs for MUC-1 Antibody (NB120-15481)

Learn more about PTMs related to MUC-1 Antibody (NB120-15481).
 

Research Areas for MUC-1 Antibody (NB120-15481)

Find related products by research area.

Blogs on MUC-1.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Mucin 1 - a mucosal epithelial glycoprotein with importance in cancer diagnostics
Mucin 1 (Muc1) is a heavily glycosylated protein that coats mucosal epithelial cells of the lungs, intestines, and other organs. Muc1 is thought to protect cells by binding to pathogens and responding to infections. During trafficking to the plasma...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
05/05/2014
Application: IHC-P
Species: Mouse

Marion Pollheimer
09/15/2010
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol MUC1
Entrez
Uniprot