IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining. Predicted molecular weight: 122 kDa. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26002932)
350 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
FAQs for MUC-1 Antibody (NB120-15481). (Showing 1 - 2 of 2 FAQs).
I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.
I have a customer who is asking about whether MUC1 Partial Recombinant Protein from Novus (Cat# H00004582-Q01) can be used with MUC1 antibody (Cat# NB120-15481). Their question was "Does the MUC1 antibody NB120-15481 have a high affinity for H00004582-Q01, given that it is recombinant. I need to find the protein with higher capacity for binding to that antibody." According to the product datasheet for the has been raised against a "Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) from cytoplasmic tail of human MUC-1". The partial recombinant MUC1 protein is made of "MUC1 (NP_877418.1, 315 a.a. - 420 a.a.)". So it depends if the MUC1 sequence that the MUC1 antibody was raised against is within this partial recombinant MUC1 protein sequence. The precise amino acid sequence of the immunogen for MUC1 antibody is not listed on the datasheet due to commercial sensitivity. Can you please advise if the amino acid sequence of the MUC1 antibody immunogen is within the partial recombinant MUC1 protein? If so, do you predict the antibody will bind this recombinant protein with high affinity? If not, is there an alternative product you can suggest? Just an additional note that the customer has just clarified: The products are to be used for "developing immunoassays with biosensors so essentially I need this antibody-protein pair for optimization of detection conditions before trying detection in real samples.
Our MUC1 antibody with catalogue number NB120-15481 was raised to a synthetic peptide derived from the cytoplasmic tail of human MUC1. According to the Uniprot listing (P15941) the cytoplasmic region of MUC1 is from amino acids 1182-1255. Our partial recombinant MUC1 protein with catalogue number H00004582-Q01 is derived from amino acids 315-420 so it is unlikely that NB120-15481 would recognise this protein. Unfortunately this partial recombinant protein is the only human MUC1 protein that we sell. This protein is produced by Abnova, for whom we distribute, but having checked their website I see that they do not stock any other MUC1 proteins either. Our MUC1 antibody with catalogue number NB300-708 was raised to a synthetic peptide representing a 20 amino acid repeat sequence from Mucin 1. A 60 amino acid long peptide was conjugated to KLH and was used to immunize rabbits. The 20 amino acid repeat sequence used was VTSAPDTRPAPGSTAPPAHG. This repeat sequence is present from amino acids 61-1020, and so it is entirely possible that NB300-708 will detect H00004582-Q01. We have not ourselves tested the binding of NB300-708 to the partial recombinant protein. But this may be a good option for your customer to try.
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