MUC-1 Antibody (VU-12E1) (NBP1-42160)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC, IHC-Fr
Clone
VU-12E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

MUC-1 Antibody (VU-12E1) Summary

Immunogen
The antibody reacts with the MUC1 antigen.
Specificity
VU-12E1 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The extracellular domain of MUC1 largly consists of a highly conserved, O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of mAb VU12E1 is the 5-mer PDTRP of the MUC1 tandem repeat as established with "epitope fingerprinting". Source: A Balb/c mouse was immunized with live cells (107 cells/dose in PBS) of human breast cancer cell line ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by subcloning four times.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
VU-12E1 has been tested on frozen sections of human ovarian carcinoma tissue and in ELISA specifically binds to a BSA conjugated 60mer MUC1 tandem repeat.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1 mg/ml PBS
Preservative
0.05% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC-1 Antibody (VU-12E1)

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Background

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for MUC-1 Antibody (NBP1-42160). (Showing 1 - 1 of 1 FAQ).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Secondary Antibodies

 

Isotype Controls

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Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Mucin 1 - a mucosal epithelial glycoprotein with importance in cancer diagnostics
Mucin 1 (Muc1) is a heavily glycosylated protein that coats mucosal epithelial cells of the lungs, intestines, and other organs. Muc1 is thought to protect cells by binding to pathogens and responding to infections. During trafficking to the plasma...  Read full blog post.

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Bioinformatics

Gene Symbol MUC1
Entrez