MUC-1 Antibody (VU-11D1)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-42162
    • Availability
      Product Discontinued

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MUC-1 Antibody (VU-11D1) Summary

Immunogen
A Balb/c mouse was immunized with live cells (10^7 cells/dose in PBS) of human breast cancer cell line ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells.
Specificity
The dominant epitope of mAb VU11D1 is the 7-mer TSAPDTR of the MUC1 tandem repeat as established with "epitope fingerprinting"
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MUC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
VU-11D1 has been tested on frozen sections of human ovarian carcinoma tissue and in ELISA specifically binds to a BSA conjugated 60mer MUC1 tandem repeat. Immunoreactivity has been demonstrated with parafin sections of normal breast tissue, but very weakly with sections of small intestine and colon.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC-1 Antibody (VU-11D1)

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Background

MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for MUC-1 Antibody (NBP1-42162). (Showing 1 - 1 of 1 FAQ).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

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Bioinformatics

Gene Symbol MUC1
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