Home » MRPS9 » MRPS9 Research Products » MRPS9 293T Cell Transient Overexpression Lysate

MRPS9 293T Cell Transient Overexpression Lysate

 Submit a Review

Images

 
There are currently no images for MRPS9 Lysate (H00064965-T02).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

100% Guarantee on every product

MRPS9 293T Cell Transient Overexpression Lysate Summary

Description
Plasmid: pCMV-MRPS9 full-length
Specificity
MRPS9 293T Cell Transient Overexpression Lysate(Denatured)
Gene
MRPS9

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-MRPS9 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MRPS9 293T Cell Transient Overexpression Lysate

  • mitochondrial ribosomal protein S9
  • MRP-S9RPMS9S9mt28S ribosomal protein S9, mitochondrial

Background

Plasmid: pCMV-MRPS9 Full length; Host Cell: 293T

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

RPS9 Antibody
NBP2-88192
Immunohistochemistry-Paraffin: RPS9 Antibody [NBP2-88192] - Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver. Observed Staining: Cytoplasm in hepatocytes, strong signal, very wide tissue distribution. Primary Antibody Concentration: 1:100.
Immunohistochemistry-Paraffin: RPS9 Antibody [NBP2-88192] - Rabbit Anti-RPS9 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver. Observed Staining: Cytoplasm in hepatocytes. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy3.
Western Blot: RPS9 Antibody [NBP2-88192] - WB Suggested Anti-RPS9 Antibody. Titration: 1.0 ug/ml. Positive Control: Hela Whole CellRPS9 is supported by BioGPS gene expression data to be expressed in HeLa
Species: Hu
Applications: IHC, IHC-P, WB
PSMD11 Antibody
NBP1-46191
Western Blot: PSMD11 Antibody [NBP1-46191] - Treatment of human skin fibroblasts with 6BIO activated proteostatic modules. Representative immunoblot analyses of the autophagy-related Beclin1 and the 26S proteasome subunits Rpn6 (PSMD11), Rpt6, alpha7 and beta5 expression levels after cell exposure to the shown concentrations of 6BIO for 36 h. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-017-11662-7), licensed under a CC-BY licence.
Western Blot: PSMD11 Antibody [NBP1-46191] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PSMD11 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PSMD11 was also immunoprecipitated by rabbit anti-PSMD11 antibody NBP1-46192, which recognizes a downstream epitope.
(10 Publications)
Species: Dr, Hu, Mu
Applications: IP, WB
Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
(58 Publications)
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
AKR1A1 Antibody
(OTI6E3)
NBP2-02164
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Hela cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - Analysis of Jurkat cells, using anti-AKR1A1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: AKR1A1 Antibody (6E3) [NBP2-02164] - HEK293T cells transfected with either pCMV6-ENTRY AKR1A1.(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal, and then analyzed by flow cytometry.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Neurophysin
II/Arg-vasopressin An ...
AF009
Neurophysin II was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human Neurophysin II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF009) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Species: Hu
Applications: IHC, WB
POLR2E Antibody
(OTI3B5)
NBP2-00482
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2E antibody, and then analyzed by flow cytometry.
Western Blot: POLR2E Antibody (OTI3B5) [NBP2-00482] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.
(1 Publication)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
alcohol
dehydrogenase 5 Antib
NBP2-49350
Western Blot: alcohol dehydrogenase 5 Antibody [NBP2-49350] - Analysis in control (vector only transfected HEK293T lysate) and ADH5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: alcohol dehydrogenase 5 Antibody [NBP2-49350] - Analysis in human endometrium and skeletal muscle tissues. Corresponding ADH5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: alcohol dehydrogenase 5 Antibody [NBP2-49350] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, Simple Western, WB
alcohol
dehydrogenase 1B Anti ...
NBP2-00649
Western Blot: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of extracts (10ug) from 9 Human tissue by using anti-ADH1B monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Hela cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: alcohol dehydrogenase 1B Antibody (OTI4F12) [NBP2-00649] - Analysis of Jurkat cells, using anti-alcohol dehydrogenase 1B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Species: Ca, Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
alcohol
dehydrogenase Antibod ...
NBP2-75398
Immunocytochemistry/Immunofluorescence: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Staining Alcohol Dehydrogenase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded human liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: alcohol dehydrogenase Antibody (JG82-31) [NBP2-75398] - Analysis of paraffin-embedded mouse liver tissue using anti-Alcohol Dehydrogenase antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MRPS9 293T Cell
Transient Overexpress ...
H00064965-T02
MRPS9 293T Cell Transient Overexpression Lysate
Species: Hu
Applications: WB

Publications for MRPS9 Lysate (H00064965-T02) (0)

There are no publications for MRPS9 Lysate (H00064965-T02).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MRPS9 Lysate (H00064965-T02) (0)

There are no reviews for MRPS9 Lysate (H00064965-T02). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MRPS9 Lysate (H00064965-T02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MRPS9 Products

Bioinformatics Tool for MRPS9 Lysate (H00064965-T02)

Discover related pathways, diseases and genes to MRPS9 Lysate (H00064965-T02). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on MRPS9

There are no specific blogs for MRPS9, but you can read our latest blog posts.

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MRPS9 293T Cell Transient Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol MRPS9