Mouse IgM Native Protein

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SDS-Page: Mouse IgM Native Protein [NBP1-96975] - SDS-PAGE of Mouse IgM Kappa isotype control. Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF, IP, PAGE
Concentration
1.0 mg/ml

Order Details

Mouse IgM Native Protein Summary

Description
Native IGHM protein.

Source: Mouse Serum

Preparation
Method
Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3 and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Light and heavy chain composition confirmed by Elisa.
Protein/Peptide Type
Native Protein
Gene
IGHM
Purity
Immunoelectrophoresis (IEP)

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunoelectrophoresis (IEP)

Alternate Names for Mouse IgM Native Protein

  • constant region of heavy chain of IgM
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • Hepatitis B virus receptor binding protein
  • Ig mu chain C region
  • IGHM
  • Immunoglobin heavy constant mu
  • Immunoglobulin heavy constant gamma 3
  • Immunoglobulin heavy mu chain
  • Immunoglobulin mu chain
  • immunoglobulin mu
  • Imunoglobulin heavy chain
  • MGC104996
  • MGC52291
  • mu
  • -region CH4 membrane bound domain
  • VH

Background

Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and u - IgM, light chain kappa and lambda.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGHM