Rat anti-Mouse Mouse IgM Secondary Antibody (eB121-15F9) [PE]

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Flow Cytometry: Mouse IgM Antibody (eB121-15F9) [PE] [NBP1-43749] - See notes for image caption

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
eB121-15F9
Clonality
Monoclonal
Host
Rat
Conjugate
PE
Concentration
0.2 mg/ml

Order Details

Rat anti-Mouse Mouse IgM Secondary Antibody (eB121-15F9) [PE] Summary

Immunogen
The immunogen for this antibody was PE IgM.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IGHM
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Rat anti-Mouse Mouse IgM Secondary Antibody (eB121-15F9) [PE]

  • constant region of heavy chain of IgM
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • Hepatitis B virus receptor binding protein
  • Ig mu chain C region
  • IGHM
  • Immunoglobin heavy constant mu
  • Immunoglobulin heavy constant gamma 3
  • Immunoglobulin heavy mu chain
  • Immunoglobulin mu chain
  • immunoglobulin mu
  • Imunoglobulin heavy chain
  • MGC104996
  • MGC52291
  • mu
  • -region CH4 membrane bound domain
  • VH

Background

The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGHM